1V0F
Endosialidase of Bacteriophage K1F in complex with oligomeric alpha-2,8-sialic acid
- PDB DOI: https://doi.org/10.2210/pdb1V0F/pdb
- Classification: HYDROLASE
- Organism(s): Escherichia phage K1F
- Expression System: Escherichia coli
- Mutation(s): No 
- Deposited: 2004-03-28 Released: 2004-12-13 
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.55 Å
- R-Value Free: 0.232 
- R-Value Work: 0.180 
- R-Value Observed: 0.183 
This is version 2.0 of the entry. See complete history. 
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
---|---|---|---|---|---|
Molecule | Chains | Sequence Length | Organism | Details | Image |
ENDO-ALPHA-SIALIDASE | 666 | Escherichia phage K1F | Mutation(s): 0  EC: 3.2.1.129 | ||
UniProt | |||||
Find proteins for Q04830 (Escherichia phage K1F) Explore Q04830  Go to UniProtKB:  Q04830 | |||||
Entity Groups   | |||||
Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
UniProt Group | Q04830 | ||||
Sequence AnnotationsExpand | |||||
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Oligosaccharides
Small Molecules
Ligands 2 Unique | |||||
---|---|---|---|---|---|
ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
SLB Query on SLB | J [auth A] L [auth B] N [auth C] P [auth D] R [auth E] | N-acetyl-beta-neuraminic acid C11 H19 N O9 SQVRNKJHWKZAKO-PFQGKNLYSA-N | |||
PO4 Query on PO4 | K [auth A] M [auth B] O [auth C] Q [auth D] T [auth F] | PHOSPHATE ION O4 P NBIIXXVUZAFLBC-UHFFFAOYSA-K |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.55 Å
- R-Value Free: 0.232 
- R-Value Work: 0.180 
- R-Value Observed: 0.183 
- Space Group: P 2 2 21
Unit Cell:
Length ( Å ) | Angle ( ˚ ) |
---|---|
a = 99.54 | α = 90 |
b = 131.4 | β = 90 |
c = 346.04 | γ = 90 |
Software Name | Purpose |
---|---|
REFMAC | refinement |
MOSFLM | data reduction |
SCALA | data scaling |
Entry History 
Deposition Data
- Released Date: 2004-12-13  Deposition Author(s): Stummeyer, K., Dickmanns, A., Muehlenhoff, M., Gerady-Schahn, R., Ficner, R.
Revision History (Full details and data files)
- Version 1.0: 2004-12-13
Type: Initial release - Version 1.1: 2011-05-07
Changes: Version format compliance - Version 1.2: 2011-07-13
Changes: Version format compliance - Version 2.0: 2020-07-29
Type: Remediation
Reason: Carbohydrate remediation
Changes: Atomic model, Data collection, Derived calculations, Other, Structure summary