1SCM

STRUCTURE OF THE REGULATORY DOMAIN OF SCALLOP MYOSIN AT 2.8 ANGSTROMS RESOLUTION


Experimental Data Snapshot

  • Method: X-RAY DIFFRACTION
  • Resolution: 2.80 Å
  • R-Value Work: 0.201 
  • R-Value Observed: 0.201 

wwPDB Validation   3D Report Full Report


This is version 1.4 of the entry. See complete history


Literature

Structure of the regulatory domain of scallop myosin at 2.8 A resolution.

Xie, X.Harrison, D.H.Schlichting, I.Sweet, R.M.Kalabokis, V.N.Szent-Gyorgyi, A.G.Cohen, C.

(1994) Nature 368: 306-312

  • DOI: https://doi.org/10.1038/368306a0
  • Primary Citation of Related Structures:  
    1SCM

  • PubMed Abstract: 

    The regulatory domain of scallop myosin is a three-chain protein complex that switches on this motor in response to Ca2+ binding. This domain has been crystallized and the structure solved to 2.8 A resolution. Side-chain interactions link the two light chains in tandem to adjacent segments of the heavy chain bearing the IQ-sequence motif. The Ca(2+)-binding site is a novel EF-hand motif on the essential light chain and is stabilized by linkages involving the heavy chain and both light chains, accounting for the requirement of all three chains for Ca2+ binding and regulation in the intact myosin molecule.


  • Organizational Affiliation

    Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, Massachusetts 02254-9110.


Macromolecules
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Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
MYOSIN HEAVY CHAIN60Argopecten irradiansMutation(s): 0 
UniProt
Find proteins for P24733 (Argopecten irradians)
Explore P24733 
Go to UniProtKB:  P24733
Entity Groups  
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UniProt GroupP24733
Sequence Annotations
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  • Reference Sequence
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Entity ID: 2
MoleculeChains Sequence LengthOrganismDetailsImage
MYOSIN REGULATORY LIGHT CHAIN145Argopecten irradiansMutation(s): 0 
UniProt
Find proteins for P13543 (Argopecten irradians)
Explore P13543 
Go to UniProtKB:  P13543
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UniProt GroupP13543
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  • Reference Sequence
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Entity ID: 3
MoleculeChains Sequence LengthOrganismDetailsImage
MYOSIN ESSENTIAL LIGHT CHAIN149Argopecten irradiansMutation(s): 0 
UniProt
Find proteins for P07291 (Argopecten irradians)
Explore P07291 
Go to UniProtKB:  P07291
Entity Groups  
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UniProt GroupP07291
Sequence Annotations
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  • Reference Sequence
Small Molecules
Ligands 1 Unique
IDChains Name / Formula / InChI Key2D Diagram3D Interactions
CA
Query on CA

Download Ideal Coordinates CCD File 
D [auth B],
E [auth C]
CALCIUM ION
Ca
BHPQYMZQTOCNFJ-UHFFFAOYSA-N
Experimental Data & Validation

Experimental Data

  • Method: X-RAY DIFFRACTION
  • Resolution: 2.80 Å
  • R-Value Work: 0.201 
  • R-Value Observed: 0.201 
  • Space Group: P 1 21 1
Unit Cell:
Length ( Å )Angle ( ˚ )
a = 52.5α = 90
b = 87β = 114.5
c = 55.5γ = 90
Software Package:
Software NamePurpose
X-PLORmodel building
X-PLORrefinement
X-PLORphasing

Structure Validation

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Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 1994-04-30
    Type: Initial release
  • Version 1.1: 2008-03-24
    Changes: Version format compliance
  • Version 1.2: 2011-07-13
    Changes: Version format compliance
  • Version 1.3: 2018-04-04
    Changes: Data collection, Other
  • Version 1.4: 2024-02-14
    Changes: Data collection, Database references, Derived calculations