Download MendeleyInsights in the catalytic mechanism of AphA from Escherichia coli
Calderone, V., Forleo, C., Benvenuti, M., Rossolini, G.M., Thaller, M.C., Mangani, S.To be published.
1RMT
Crystal structure of AphA class B acid phosphatase/phosphotransferase complexed with adenosine.
- PDB DOI: https://doi.org/10.2210/pdb1RMT/pdb
- Classification: HYDROLASE
- Organism(s): Escherichia coli
- Expression System: Escherichia coli
- Mutation(s): No
- Deposited: 2003-11-28 Released: 2004-12-14
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.40 Å
- R-Value Free: 0.199
- R-Value Work: 0.167
- R-Value Observed: 0.169
This is version 1.3 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Class B acid phosphatase | 212 | Escherichia coli | Mutation(s): 0 Gene Names: APHA, NAPA, B4055, SF4149, S3580 EC: 3.1.3.2 |  | |
UniProt | |||||
Find proteins for P0AE22 (Escherichia coli (strain K12)) Explore P0AE22 Go to UniProtKB: P0AE22 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P0AE22 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| ADN Query on ADN | F [auth A], H [auth B], J [auth C], L [auth D] | ADENOSINE C10 H13 N5 O4 OIRDTQYFTABQOQ-KQYNXXCUSA-N |  | ||
| MG Query on MG | E [auth A], G [auth B], I [auth C], K [auth D] | MAGNESIUM ION Mg JLVVSXFLKOJNIY-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.40 Å
- R-Value Free: 0.199
- R-Value Work: 0.167
- R-Value Observed: 0.169
- Space Group: P 1 21 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 84.74 | α = 90 |
| b = 66.704 | β = 117.13 |
| c = 88.556 | γ = 90 |
| Software Name | Purpose |
|---|---|
| REFMAC | refinement |
| MOSFLM | data reduction |
| CCP4 | data scaling |
| MOLREP | phasing |
Entry History
Deposition Data
- Released Date: 2004-12-14 Deposition Author(s): Calderone, V., Forleo, C., Benvenuti, M., Rossolini, G.M., Thaller, M.C., Mangani, S.
Revision History (Full details and data files)
- Version 1.0: 2004-12-14
Type: Initial release - Version 1.1: 2008-04-29
Changes: Version format compliance - Version 1.2: 2011-07-13
Changes: Derived calculations, Version format compliance - Version 1.3: 2023-08-23
Changes: Data collection, Database references, Derived calculations, Refinement description
