Download MendeleyThe structure of the DNA binding protein HU Thermotoga maritima with a single conservative substitution (V42I) that confers significant destabilisation
Kavounis, C., Petratos, K., Tucker, P., Vorgias, C.E.To be published.
1RIY
HU mutant V42I from Thermotoga maritima
- PDB DOI: https://doi.org/10.2210/pdb1RIY/pdb
- Classification: DNA BINDING PROTEIN
- Organism(s): Thermotoga maritima
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): Yes
- Deposited: 2003-11-18 Released: 2004-12-07
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.80 Å
- R-Value Free: 0.286
- R-Value Work: 0.216
- R-Value Observed: 0.219
wwPDB Validation 3D Report Full Report
This is version 1.5 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Hu DNA-binding protein | 90 | Thermotoga maritima | Mutation(s): 1 Gene Names: HUP |  | |
UniProt | |||||
Find proteins for P36206 (Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)) Explore P36206 Go to UniProtKB: P36206 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P36206 | ||||
Sequence AnnotationsExpand | |||||
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Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.80 Å
- R-Value Free: 0.286
- R-Value Work: 0.216
- R-Value Observed: 0.219
- Space Group: P 43 21 2
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 45.447 | α = 90 |
| b = 45.447 | β = 90 |
| c = 76.204 | γ = 90 |
| Software Name | Purpose |
|---|---|
| DENZO | data reduction |
| SCALEPACK | data scaling |
| AMoRE | phasing |
| REFMAC | refinement |
Entry History
Deposition Data
- Released Date: 2004-12-07 Deposition Author(s): Kavounis, C., Petratos, K., Tucker, P., Vorgias, C.E.
Revision History (Full details and data files)
- Version 1.0: 2004-12-07
Type: Initial release - Version 1.1: 2008-04-29
Changes: Version format compliance - Version 1.2: 2011-07-13
Changes: Advisory, Derived calculations, Version format compliance - Version 1.3: 2017-10-11
Changes: Refinement description - Version 1.4: 2021-11-10
Changes: Data collection, Database references - Version 1.5: 2023-10-25
Changes: Data collection, Refinement description
