Download MendeleyCrystal Structure of Cambialistic Superoxide Dismutase from Porphyromonas Gingivalis
Sugio, S., Hiraoka, B.Y., Yamakura, F.(2000) Eur J Biochem 267: 3487
- PubMed: 10848964
- DOI: https://doi.org/10.1046/j.1432-1327.2000.01373.x
- Primary Citation of Related Structures:
1QNN - PubMed Abstract:
The crystal structure of cambialistic superoxide dismutase (SOD) from Porphyromonas gingivalis, which exhibits full activity with either Fe or Mn at the active site, has been determined at 1.8-A resolution by molecular replacement and refined to a crystallographic R factor of 17.9% (Rfree 22.3%). The crystals belong to the space group P212121 (a = 75.5 A, b = 102.7 A, c = 99.6 A) with four identical subunits in the asymmetric unit. Each pair of subunits forms a compact dimer, but not a tetramer, with 222 point symmetry. Each subunit has 191 amino-acid residues most of which are visible in electron density maps, and consists of seven alpha helices and one three-stranded antiparallel beta sheet. The metal ion, a 3 : 1 mixture of Fe and Mn, is coordinated with five ligands (His27, His74, His161, Asp157, and water) arranged at the vertices of a trigonal bipyramid. Although the overall structural features, including the metal coordination geometry, are similar to those found in other single-metal containing SODs, P. gingivalis SOD more closely resembles the dimeric Fe-SODs from Escherichia coli rather than another cambialistic SOD from Propionibacterium shermanii, which itself is rather similar to other tetrameric SODs.
Organizational Affiliation:
Yokohama Research Center, Mitsubishi Chemical Corporation, Aoba, Yokohama, Japan.
