Download MendeleyThe Structure of L-2-Haloacid Dehalogenase Complexed with a Reaction Product Reveals the Mechanism of Intermediate Hydrolysis in Dehalogenase
Li, Y.-F., Hata, Y., Fujii, T., Kurihara, T., Esaki, N.To be published.
1QH9
ENZYME-PRODUCT COMPLEX OF L-2-HALOACID DEHALOGENASE
- PDB DOI: https://doi.org/10.2210/pdb1QH9/pdb
- Classification: HYDROLASE
- Organism(s): Pseudomonas sp. YL
- Expression System: Escherichia coli
- Mutation(s): No
- Deposited: 1999-05-12 Released: 2000-05-24
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.50 Å
- R-Value Free: 0.287
- R-Value Work: 0.194
wwPDB Validation 3D Report Full Report
This is version 2.0 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| 2-HALOACID DEHALOGENASE | 232 | Pseudomonas sp. YL | Mutation(s): 0 Gene Names: DEX YL EC: 3.8.1.2 |  | |
UniProt | |||||
Find proteins for Q53464 (Pseudomonas sp. (strain YL)) Explore Q53464 Go to UniProtKB: Q53464 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q53464 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| LAC Query on LAC | B [auth A] | LACTIC ACID C3 H6 O3 JVTAAEKCZFNVCJ-UWTATZPHSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.50 Å
- R-Value Free: 0.287
- R-Value Work: 0.194
- Space Group: C 1 2 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 91.81 | α = 90 |
| b = 62.76 | β = 123.07 |
| c = 51.38 | γ = 90 |
| Software Name | Purpose |
|---|---|
| X-PLOR | model building |
| X-PLOR | refinement |
| PROCESS | data reduction |
| PROCESS | data scaling |
| X-PLOR | phasing |
Entry History
Deposition Data
- Released Date: 2000-05-24 Deposition Author(s): Li, Y.-F., Hata, Y., Fujii, T., Kurihara, T., Esaki, N.
Revision History (Full details and data files)
- Version 1.0: 2000-05-24
Type: Initial release - Version 1.1: 2008-04-26
Changes: Version format compliance - Version 1.2: 2011-07-13
Changes: Derived calculations, Source and taxonomy, Version format compliance - Version 2.0: 2023-11-15
Changes: Atomic model, Data collection, Database references, Derived calculations
