1Q7F

Model of the Brain Tumor-Pumilio translation repressor complex

(2003) Genes Dev 17: 2508-2513

• PubMed: 14561773 Search on PubMedSearch on PubMed Central
• DOI: https://doi.org/10.1101/gad.1119403
• Primary Citation of Related Structures:  
  1Q7F


• PubMed Abstract: 
  

  The Brain Tumor (Brat) protein is recruited to the 3' untranslated region (UTR) of hunchback mRNA to regulate its translation. Recruitment is mediated by interactions between the Pumilio RNA-binding Puf repeats and the NHL domain of Brat, a conserved structural motif present in a large family of growth regulators. In this report, we describe the crystal structure of the Brat NHL domain and present a model of the Pumilio-Brat complex derived from in silico docking experiments and supported by mutational analysis of the protein-protein interface. A key feature of the model is recognition of the outer, convex surface of the Pumilio Puf domain by the top, electropositive face of the six-bladed Brat beta-propeller. In particular, an extended loop in Puf repeat 8 fits in the entrance to the central channel of the Brat beta-propeller. Together, these interactions are likely to be prototypic of the recruitment strategies of other NHL-containing proteins in development.

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Organizational Affiliation: 

Department of Physiology and Biophysics, Mount Sinai School of Medicine, New York, New York 10029, USA.