Download MendeleyLocking and Unlocking of Ribosomal Motions
Valle, M., Zavialov, A., Sengupta, J., Rawat, U., Ehrenberg, M., Frank, J.(2003) Cell 114: 123-134
- PubMed: 12859903
- DOI: https://doi.org/10.1016/s0092-8674(03)00476-8
- Primary Citation of Related Structures:
1PN6, 1PN7, 1PN8 - PubMed Abstract:
During the ribosomal translocation, the binding of elongation factor G (EF-G) to the pretranslocational ribosome leads to a ratchet-like rotation of the 30S subunit relative to the 50S subunit in the direction of the mRNA movement. By means of cryo-electron microscopy we observe that this rotation is accompanied by a 20 A movement of the L1 stalk of the 50S subunit, implying that this region is involved in the translocation of deacylated tRNAs from the P to the E site. These ribosomal motions can occur only when the P-site tRNA is deacylated. Prior to peptidyl-transfer to the A-site tRNA or peptide removal, the presence of the charged P-site tRNA locks the ribosome and prohibits both of these motions.
Organizational Affiliation:
Howard Hughes Medical Institute, and Health Research Incororated at the Wadswoth Center, State University of New York, Albany, 12201, USA.
