1OA2
Comparison of Family 12 Glycoside Hydrolases and Recruited Substitutions Important for Thermal Stability
- PDB DOI: https://doi.org/10.2210/pdb1OA2/pdb
- Classification: HYDROLASE
- Organism(s): Trichoderma reesei
- Expression System: Aspergillus niger
- Mutation(s): Yes
- Deposited: 2002-12-28 Released: 2003-03-27
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.50 Å
- R-Value Free: 0.223
- R-Value Work: 0.205
- R-Value Observed: 0.206
This is version 2.2 of the entry. See complete history.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| ENDO-BETA-1,4-GLUCANASE | 218 | Trichoderma reesei | Mutation(s): 2 EC: 3.2.1.4 |  | |
UniProt | |||||
Find proteins for O00095 (Hypocrea jecorina) Explore O00095 Go to UniProtKB: O00095 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | O00095 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| NAG Query on NAG | G [auth A] H [auth B] I [auth C] J [auth D] K [auth E] | 2-acetamido-2-deoxy-beta-D-glucopyranose C8 H15 N O6 OVRNDRQMDRJTHS-FMDGEEDCSA-N |  | ||
| Modified Residues 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Type | Formula | 2D Diagram | Parent |
| PCA Query on PCA | A, B, C, D, E A, B, C, D, E, F | L-PEPTIDE LINKING | C5 H7 N O3 |  | GLN |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.50 Å
- R-Value Free: 0.223
- R-Value Work: 0.205
- R-Value Observed: 0.206
- Space Group: P 1 21 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 68.28 | α = 90 |
| b = 71.29 | β = 91.49 |
| c = 119.3 | γ = 90 |
| Software Name | Purpose |
|---|---|
| REFMAC | refinement |
| DENZO | data reduction |
| SCALEPACK | data scaling |
| AMoRE | phasing |
Entry History
Deposition Data
- Released Date: 2003-03-27 Deposition Author(s): Sandgren, M., Gualfetti, P.J., Shaw, A., Gross, L.S., Saldajeno, M., Day, A.G., Jones, T.A., Mitchinson, C.
Revision History (Full details and data files)
- Version 1.0: 2003-03-27
Type: Initial release - Version 1.1: 2011-05-08
Changes: Version format compliance - Version 1.2: 2011-07-13
Changes: Version format compliance - Version 2.0: 2020-03-11
Changes: Data collection, Derived calculations, Other, Polymer sequence - Version 2.1: 2020-07-29
Type: Remediation
Reason: Carbohydrate remediation
Changes: Data collection, Derived calculations, Structure summary - Version 2.2: 2023-12-13
Changes: Data collection, Database references, Refinement description, Structure summary
