1O08
Structure of Pentavalent Phosphorous Intermediate of an Enzyme Catalyzed Phosphoryl transfer Reaction observed on cocrystallization with Glucose 1-phosphate
- PDB DOI: https://doi.org/10.2210/pdb1O08/pdb
- Classification: ISOMERASE
- Organism(s): Lactococcus lactis
- Expression System: Escherichia coli BL21
- Mutation(s): No
- Deposited: 2003-02-20 Released: 2003-03-18
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.20 Å
- R-Value Free: 0.179
- R-Value Work: 0.141
- R-Value Observed: 0.141
This is version 2.0 of the entry. See complete history.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| beta-phosphoglucomutase | 221 | Lactococcus lactis | Mutation(s): 0 EC: 5.4.2.6 |  | |
UniProt | |||||
Find proteins for P71447 (Lactococcus lactis subsp. lactis (strain IL1403)) Explore P71447 Go to UniProtKB: P71447 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P71447 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| G16 Query on G16 | B [auth A] | 1,6-di-O-phosphono-alpha-D-glucopyranose C6 H13 O12 P2 RWHOZGRAXYWRNX-VFUOTHLCSA-M |  | ||
| MG Query on MG | C [auth A] | MAGNESIUM ION Mg JLVVSXFLKOJNIY-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.20 Å
- R-Value Free: 0.179
- R-Value Work: 0.141
- R-Value Observed: 0.141
- Space Group: P 21 21 21
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 36.939 | α = 90 |
| b = 54.297 | β = 90 |
| c = 104.68 | γ = 90 |
| Software Name | Purpose |
|---|---|
| SHELX | model building |
| SHELXL-97 | refinement |
| DENZO | data reduction |
| SCALEPACK | data scaling |
| SHELX | phasing |
Entry History
Deposition Data
- Released Date: 2003-03-18 Deposition Author(s): Lahiri, S.D., Zhang, G., Dunaway-Mariano, D., Allen, K.N.
Revision History (Full details and data files)
- Version 1.0: 2003-03-18
Type: Initial release - Version 1.1: 2008-04-29
Changes: Version format compliance - Version 1.2: 2011-07-13
Changes: Version format compliance - Version 1.3: 2020-07-29
Type: Remediation
Reason: Carbohydrate remediation
Changes: Advisory, Data collection, Derived calculations, Structure summary - Version 2.0: 2024-04-03
Changes: Atomic model, Data collection, Database references, Derived calculations, Refinement description, Structure summary
