1NTP

USE OF THE NEUTRON DIFFRACTION H/D EXCHANGE TECHNIQUE TO DETERMINE THE CONFORMATIONAL DYNAMICS OF TRYPSIN

PDB DOI: https://doi.org/10.2210/pdb1NTP/pdb

Classification: HYDROLASE (SERINE PROTEINASE)
Organism(s): Bos taurus
Mutation(s): No

Deposited: 1987-09-16 Released: 1988-01-16
Deposition Author(s): Kossiakoff, A.A.

Experimental Data Snapshot

Method: NEUTRON DIFFRACTION
Resolution: 1.80 Å
R-Value Work: 0.187

wwPDB Validation 3D Report Full Report

This is version 1.3 of the entry. See complete history.

Literature

Use of the neutron diffraction--H/D exchange technique to determine the conformational dynamics of trypsin

Kossiakoff, A.A.

(1984) Basic Life Sci 27: 281-304

PubMed: 6712567 Search on PubMed
DOI: https://doi.org/10.1007/978-1-4899-0375-4_17
Primary Citation of Related Structures:
1NTP

Macromolecule Content

Total Structure Weight: 23.47 kDa
Atom Count: 1,636
Modelled Residue Count: 223
Deposited Residue Count: 223
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
BETA-TRYPSIN	A	223	Bos taurus	Mutation(s): 0 EC: 3.4.21.4
UniProt
Find proteins for P00760 (Bos taurus) Explore P00760 Go to UniProtKB: P00760
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	P00760
Sequence Annotations Expand
Reference Sequence

Small Molecules

Ligands 1 Unique
ID	Chains	Name / Formula / InChI Key	2D Diagram	3D Interactions
ISP Query on ISP Download Ideal Coordinates CCD File SDF format, chain B [auth A] MOL2 format, chain B [auth A]	B [auth A]	PHOSPHORYLISOPROPANE C₃ H₉ O₄ P QPPQHRDVPBTVEV-UHFFFAOYSA-N		Interactions Focus chain B [auth A]

Experimental Data & Validation

Experimental Data

Method: NEUTRON DIFFRACTION
Resolution: 1.80 Å
R-Value Work: 0.187
Space Group: P 2₁ 2₁ 2₁

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 54.84	α = 90
b = 58.61	β = 90
c = 67.47	γ = 90

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 1988-01-16

Deposition Author(s):

Kossiakoff, A.A.

Revision History (Full details and data files)

Version 1.0: 1988-01-16
Type: Initial release
Version 1.1: 2008-03-03
Changes: Version format compliance
Version 1.2: 2011-07-13
Changes: Version format compliance
Version 1.3: 2017-11-29
Changes: Derived calculations, Other

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.