1NMM
beta-1,4-galactosyltransferase mutant Cys342Thr complex with alpha-lactalbumin and GlcNAc
- PDB DOI: https://doi.org/10.2210/pdb1NMM/pdb
- Entry: 1NMM supersedes: 1JN8
- Classification: TRANSFERASE ACTIVATOR/TRANSFERASE
- Organism(s): Mus musculus, Bos taurus
- Expression System: Escherichia coli
- Mutation(s): Yes 
- Deposited: 2003-01-10 Released: 2003-01-21 
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.00 Å
- R-Value Free: 0.229 
- R-Value Work: 0.189 
- R-Value Observed: 0.189 
This is version 1.4 of the entry. See complete history. 
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
---|---|---|---|---|---|
Molecule | Chains | Sequence Length | Organism | Details | Image |
ALPHA-LACTALBUMIN | 123 | Mus musculus | Mutation(s): 0  | ||
UniProt | |||||
Find proteins for P29752 (Mus musculus) Explore P29752  Go to UniProtKB:  P29752 | |||||
Entity Groups   | |||||
Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
UniProt Group | P29752 | ||||
Sequence AnnotationsExpand | |||||
|
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 2 | |||||
---|---|---|---|---|---|
Molecule | Chains | Sequence Length | Organism | Details | Image |
BETA-1,4-GALACTOSYLTRANSFERASE | 286 | Bos taurus | Mutation(s): 1  EC: 2.4.1.22 (PDB Primary Data), 2.4.1.90 (PDB Primary Data), 2.4.1.38 (PDB Primary Data) | ||
UniProt | |||||
Find proteins for P08037 (Bos taurus) Explore P08037  Go to UniProtKB:  P08037 | |||||
Entity Groups   | |||||
Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
UniProt Group | P08037 | ||||
Sequence AnnotationsExpand | |||||
|
Small Molecules
Ligands 3 Unique | |||||
---|---|---|---|---|---|
ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
NAG Query on NAG | F [auth B], H [auth D] | 2-acetamido-2-deoxy-beta-D-glucopyranose C8 H15 N O6 OVRNDRQMDRJTHS-FMDGEEDCSA-N | |||
PG4 Query on PG4 | I [auth D], J [auth D] | TETRAETHYLENE GLYCOL C8 H18 O5 UWHCKJMYHZGTIT-UHFFFAOYSA-N | |||
CA Query on CA | E [auth A], G [auth C] | CALCIUM ION Ca BHPQYMZQTOCNFJ-UHFFFAOYSA-N |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.00 Å
- R-Value Free: 0.229 
- R-Value Work: 0.189 
- R-Value Observed: 0.189 
- Space Group: P 1 21 1
Unit Cell:
Length ( Å ) | Angle ( ˚ ) |
---|---|
a = 57.019 | α = 90 |
b = 95.626 | β = 101.37 |
c = 100.213 | γ = 90 |
Software Name | Purpose |
---|---|
DENZO | data reduction |
SCALEPACK | data scaling |
AMoRE | phasing |
CNS | refinement |
Entry History 
Deposition Data
- Released Date: 2003-01-21  Deposition Author(s): Ramakrishnan, B., Shah, P.S., Qasba, P.K.
- This entry supersedes: 1JN8
Revision History (Full details and data files)
- Version 1.0: 2003-01-21
Type: Initial release - Version 1.1: 2008-04-29
Changes: Version format compliance - Version 1.2: 2011-07-13
Changes: Non-polymer description, Version format compliance - Version 1.3: 2020-07-29
Type: Remediation
Reason: Carbohydrate remediation
Changes: Data collection, Derived calculations, Structure summary - Version 1.4: 2021-10-27
Changes: Database references, Structure summary