1NE7
HUMAN GLUCOSAMINE-6-PHOSPHATE DEAMINASE ISOMERASE AT 1.75 A RESOLUTION COMPLEXED WITH N-ACETYL-GLUCOSAMINE-6-PHOSPHATE AND 2-DEOXY-2-AMINO-GLUCITOL-6-PHOSPHATE
- PDB DOI: https://doi.org/10.2210/pdb1NE7/pdb
- Entry: 1NE7 supersedes: 1D9T
- Classification: HYDROLASE
- Organism(s): Homo sapiens
- Expression System: Escherichia coli
- Mutation(s): No 
- Deposited: 2002-12-10 Released: 2003-09-23 
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.75 Å
- R-Value Free: 0.217 
- R-Value Work: 0.193 
- R-Value Observed: 0.195 
This is version 2.2 of the entry. See complete history. 
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
---|---|---|---|---|---|
Molecule | Chains | Sequence Length | Organism | Details | Image |
Glucosamine-6-phosphate isomerase | 289 | Homo sapiens | Mutation(s): 0  Gene Names: GNPI OR HLN OR KIAA0060 EC: 3.5.99.6 | ||
UniProt & NIH Common Fund Data Resources | |||||
Find proteins for P46926 (Homo sapiens) Explore P46926  Go to UniProtKB:  P46926 | |||||
PHAROS:  P46926 GTEx:  ENSG00000113552  | |||||
Entity Groups   | |||||
Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
UniProt Group | P46926 | ||||
Sequence AnnotationsExpand | |||||
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Oligosaccharides
Small Molecules
Ligands 3 Unique | |||||
---|---|---|---|---|---|
ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
16G Query on 16G | CA [auth F] M [auth A] P [auth B] S [auth C] W [auth D] | 2-acetamido-2-deoxy-6-O-phosphono-alpha-D-glucopyranose C8 H16 N O9 P BRGMHAYQAZFZDJ-PVFLNQBWSA-N | |||
AGP Query on AGP | BA [auth E] EA [auth F] O [auth A] R [auth B] V [auth C] | 2-DEOXY-2-AMINO GLUCITOL-6-PHOSPHATE C6 H16 N O8 P LBNVXZROMBUNNQ-SLPGGIOYSA-N | |||
SO4 Query on SO4 | AA [auth E] DA [auth F] N [auth A] Q [auth B] T [auth C] | SULFATE ION O4 S QAOWNCQODCNURD-UHFFFAOYSA-L |
Biologically Interesting Molecules (External Reference) 1 Unique
Entity ID: 2 | |||||
---|---|---|---|---|---|
ID | Chains | Name | Type/Class | 2D Diagram | 3D Interactions |
PRD_900006 Query on PRD_900006 | G, H, I, J, K | trehalose | Oligosaccharide / Nutrient |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.75 Å
- R-Value Free: 0.217 
- R-Value Work: 0.193 
- R-Value Observed: 0.195 
- Space Group: P 21 21 21
Unit Cell:
Length ( Å ) | Angle ( ˚ ) |
---|---|
a = 109.877 | α = 90 |
b = 110.892 | β = 90 |
c = 180.881 | γ = 90 |
Software Name | Purpose |
---|---|
DENZO | data reduction |
SCALA | data scaling |
CNS | refinement |
CCP4 | data scaling |
CNS | phasing |
Entry History 
Deposition Data
- Released Date: 2003-09-23  Deposition Author(s): Arreola, R., Valderrama, B., Morante, M.L., Horjales, E.
- This entry supersedes: 1D9T
Revision History (Full details and data files)
- Version 1.0: 2003-09-23
Type: Initial release - Version 1.1: 2008-04-28
Changes: Version format compliance - Version 1.2: 2011-07-13
Changes: Version format compliance - Version 2.0: 2020-07-29
Type: Remediation
Reason: Carbohydrate remediation
Changes: Advisory, Atomic model, Data collection, Derived calculations, Structure summary - Version 2.1: 2020-10-14
Changes: Structure summary - Version 2.2: 2023-08-16
Changes: Advisory, Data collection, Database references, Refinement description