The 1.6 A crystal structure of E. coli argininosuccinate synthetase suggests a conformational change during catalysis.
Lemke, C.T., Howell, P.L.(2001) Structure 9: 1153-1164
- PubMed: 11738042 
- DOI: https://doi.org/10.1016/s0969-2126(01)00683-9
- Primary Citation of Related Structures:  
1K92, 1K97 - PubMed Abstract: 
Argininosuccinate synthetase (AS) is the rate-limiting enzyme of both the urea and arginine-citrulline cycles. In mammals, deficiency of AS leads to citrullinemia, a debilitating and often fatal autosomal recessive urea cycle disorder, whereas its overexpression for sustained nitric oxide production via the arginine-citrulline cycle leads to the potentially fatal hypotension associated with septic and cytokine-induced circulatory shock.
Organizational Affiliation: 
Structural Biology and Biochemistry, Research Institute, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada.