Download MendeleyCrystal Structure of Low-specificity Threonine Aldolase, a Key Enzyme in Glycine Biosynthesis
Kielkopf, C.L., Bonanno, J., Ray, S., Burley, S.K.To be published.
1JG8
Crystal Structure of Threonine Aldolase (Low-specificity)
- PDB DOI: https://doi.org/10.2210/pdb1JG8/pdb
- Classification: LYASE
- Organism(s): Thermotoga maritima
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): Yes
- Deposited: 2001-06-23 Released: 2001-07-04
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.80 Å
- R-Value Free: 0.222
- R-Value Work: 0.207
- R-Value Observed: 0.208
wwPDB Validation 3D Report Full Report
This is version 1.4 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| L-allo-threonine aldolase | 347 | Thermotoga maritima | Mutation(s): 1 Gene Names: TM1744 EC: 4.1.2.5 |  | |
UniProt | |||||
Find proteins for Q9X266 (Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)) Explore Q9X266 Go to UniProtKB: Q9X266 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q9X266 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| CA Query on CA | E [auth A] G [auth B] H [auth B] I [auth C] K [auth D] | CALCIUM ION Ca BHPQYMZQTOCNFJ-UHFFFAOYSA-N |  | ||
| NA Query on NA | F [auth A], J [auth C] | SODIUM ION Na FKNQFGJONOIPTF-UHFFFAOYSA-N |  | ||
| Modified Residues 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Type | Formula | 2D Diagram | Parent |
| LLP Query on LLP | A, B, C, D | L-PEPTIDE LINKING | C14 H22 N3 O7 P |  | LYS |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.80 Å
- R-Value Free: 0.222
- R-Value Work: 0.207
- R-Value Observed: 0.208
- Space Group: P 21 21 21
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 95.89 | α = 90 |
| b = 100.6 | β = 90 |
| c = 149.82 | γ = 90 |
| Software Name | Purpose |
|---|---|
| SnB | phasing |
| MLPHARE | phasing |
| DM | model building |
| CNS | refinement |
| DENZO | data reduction |
| SCALEPACK | data scaling |
| DM | phasing |
Entry History
Deposition Data
- Released Date: 2001-07-04 Deposition Author(s): Kielkopf, C.L., Bonanno, J., Ray, S., Burley, S.K., New York SGX Research Center for Structural Genomics (NYSGXRC)
Revision History (Full details and data files)
- Version 1.0: 2001-07-04
Type: Initial release - Version 1.1: 2008-04-27
Changes: Version format compliance - Version 1.2: 2011-07-13
Changes: Version format compliance - Version 1.3: 2017-10-04
Changes: Refinement description - Version 1.4: 2021-02-03
Changes: Database references, Derived calculations, Structure summary
