1I78
CRYSTAL STRUCTURE OF OUTER MEMBRANE PROTEASE OMPT FROM ESCHERICHIA COLI
- PDB DOI: https://doi.org/10.2210/pdb1I78/pdb
- Classification: HYDROLASE
- Organism(s): Escherichia coli K-12
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): Yes
- Membrane Protein: Yes OPMPDBTMMemProtMDmpstruc
- Deposited: 2001-03-08 Released: 2001-10-03
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.60 Å
- R-Value Free: 0.280
- R-Value Work: 0.238
This is version 1.5 of the entry. See complete history.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| PROTEASE VII | 297 | Escherichia coli K-12 | Mutation(s): 3 Gene Names: OMPT EC: 3.4.21.87 Membrane Entity: Yes |  | |
UniProt | |||||
Find proteins for P09169 (Escherichia coli (strain K12)) Explore P09169 Go to UniProtKB: P09169 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P09169 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| BOG Query on BOG | C [auth A], D [auth A], G [auth B], H [auth B] | octyl beta-D-glucopyranoside C14 H28 O6 HEGSGKPQLMEBJL-RKQHYHRCSA-N |  | ||
| MPD Query on MPD | E [auth A], F [auth A] | (4S)-2-METHYL-2,4-PENTANEDIOL C6 H14 O2 SVTBMSDMJJWYQN-YFKPBYRVSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.60 Å
- R-Value Free: 0.280
- R-Value Work: 0.238
- Space Group: P 32 2 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 98.389 | α = 90 |
| b = 98.389 | β = 90 |
| c = 165.695 | γ = 120 |
| Software Name | Purpose |
|---|---|
| MLPHARE | phasing |
| CNS | refinement |
| DENZO | data reduction |
| SCALEPACK | data scaling |
Entry History
Deposition Data
- Released Date: 2001-10-03 Deposition Author(s): Vandeputte-Rutten, L., Kramer, R.A., Kroon, J., Dekker, N., Egmond, M.R., Gros, P.
Revision History (Full details and data files)
- Version 1.0: 2001-10-03
Type: Initial release - Version 1.1: 2008-04-27
Changes: Version format compliance - Version 1.2: 2011-07-13
Changes: Derived calculations, Source and taxonomy, Version format compliance - Version 1.3: 2020-07-29
Type: Remediation
Reason: Carbohydrate remediation
Changes: Data collection, Derived calculations, Structure summary - Version 1.4: 2021-10-27
Changes: Database references, Structure summary - Version 1.5: 2024-02-07
Changes: Data collection
