1H8B

EF-hands 3,4 from alpha-actinin / Z-repeat 7 from titin


Experimental Data Snapshot

  • Method: SOLUTION NMR
  • Conformers Calculated: 50 
  • Conformers Submitted: 30 
  • Selection Criteria: LOWEST ENERGIES 

wwPDB Validation   3D Report Full Report


This is version 1.2 of the entry. See complete history


Literature

Ca2+-Independent Binding of an EF-Hand Domain to a Novel Motif in the Alpha-Actinin-Titin Complex

Atkinson, R.A.Joseph, C.Kelly, G.Muskett, F.W.Frenkiel, T.A.Nietlispach, D.Pastore, A.

(2001) Nat Struct Biol 8: 853

  • DOI: https://doi.org/10.1038/nsb1001-853
  • Primary Citation of Related Structures:  
    1H8B

  • PubMed Abstract: 

    The interaction between alpha-actinin and titin, two modular muscle proteins, is essential for sarcomere assembly. We have solved the solution structure of a complex between the calcium-insensitive C-terminal EF-hand domain of alpha-actinin-2 and the seventh Z-repeat of titin. The structure of the complex is in a semi-open conformation and closely resembles that of myosin light chains in their complexes with heavy chain IQ motifs. However, no IQ motif is present in the Z-repeat, suggesting that the semi-open conformation is a general structural solution for calcium-independent recognition of EF-hand domains.


  • Organizational Affiliation

    Division of Molecular Structure, National Institute for Medical Research, The Ridgeway, Mill Hill, London, NW7 1AA UK.


Macromolecules
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
ALPHA-ACTININ 2, SKELETAL MUSCLE ISOFORM75Homo sapiensMutation(s): 1 
UniProt & NIH Common Fund Data Resources
Find proteins for P35609 (Homo sapiens)
Explore P35609 
Go to UniProtKB:  P35609
PHAROS:  P35609
GTEx:  ENSG00000077522 
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupP35609
Sequence Annotations
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  • Reference Sequence
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 2
MoleculeChains Sequence LengthOrganismDetailsImage
TITIN53Oryctolagus cuniculusMutation(s): 1 
UniProt
Find proteins for O97791 (Oryctolagus cuniculus)
Explore O97791 
Go to UniProtKB:  O97791
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupO97791
Sequence Annotations
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: SOLUTION NMR
  • Conformers Calculated: 50 
  • Conformers Submitted: 30 
  • Selection Criteria: LOWEST ENERGIES 

Structure Validation

View Full Validation Report



Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 2001-08-30
    Type: Initial release
  • Version 1.1: 2011-05-08
    Changes: Version format compliance
  • Version 1.2: 2011-07-13
    Changes: Version format compliance