1GQQ

MURC - Crystal structure of the apo-enzyme from Haemophilus influenzae

PDB DOI: https://doi.org/10.2210/pdb1GQQ/pdb

Classification: CELL WALL BIOSYNTHESIS
Organism(s): Haemophilus influenzae
Expression System: Escherichia coli BL21
Mutation(s): No

Deposited: 2001-12-03 Released: 2003-06-15
Deposition Author(s): Skarzynski, T., Cleasby, A., Domenici, E., Gevi, M., Shaw, J.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 3.10 Å
R-Value Free: 0.286
R-Value Work: 0.239
R-Value Observed: 0.241

wwPDB Validation 3D Report Full Report

This is version 1.3 of the entry. See complete history.

Literature

Crystal Structures of Udp-N-Acetylmuramate-L-Alanine Ligase (Murc) from Haemophilus Influenzae

Skarzynski, T., Cleasby, A., Domenici, E., Gevi, M., Shaw, J.

To be published.

Macromolecule Content

Total Structure Weight: 104.11 kDa
Atom Count: 6,578
Modelled Residue Count: 855
Deposited Residue Count: 950
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
UDP-N-ACETYLMURAMATE-L-ALANINE LIGASE	A, B	475	Haemophilus influenzae	Mutation(s): 0 EC: 6.3.2.8
UniProt
Find proteins for P45066 (Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)) Explore P45066 Go to UniProtKB: P45066
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	P45066
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 3.10 Å
R-Value Free: 0.286
R-Value Work: 0.239
R-Value Observed: 0.241
Space Group: P 2₁ 2₁ 2₁

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 65.508	α = 90
b = 99.485	β = 90
c = 180.59	γ = 90

Software Package:

Software Name	Purpose
REFMAC	refinement
DENZO	data reduction
SCALEPACK	data scaling

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2003-06-15

Deposition Author(s):

Revision History (Full details and data files)

Version 1.0: 2003-06-15
Type: Initial release
Version 1.1: 2011-05-08
Changes: Version format compliance
Version 1.2: 2011-07-13
Changes: Version format compliance
Version 1.3: 2018-02-28
Changes: Source and taxonomy

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.