1F7T

HOLO-(ACYL CARRIER PROTEIN) SYNTHASE AT 1.8A


Experimental Data Snapshot

  • Method: X-RAY DIFFRACTION
  • Resolution: 1.80 Å
  • R-Value Free: 0.219 
  • R-Value Work: 0.196 
  • R-Value Observed: 0.197 

wwPDB Validation   3D Report Full Report


This is version 1.5 of the entry. See complete history


Literature

Crystal structures of substrate binding to Bacillus subtilis holo-(acyl carrier protein) synthase reveal a novel trimeric arrangement of molecules resulting in three active sites.

Parris, K.D.Lin, L.Tam, A.Mathew, R.Hixon, J.Stahl, M.Fritz, C.C.Seehra, J.Somers, W.S.

(2000) Structure 8: 883-895

  • DOI: https://doi.org/10.1016/s0969-2126(00)00178-7
  • Primary Citation of Related Structures:  
    1F7L, 1F7T, 1F80

  • PubMed Abstract: 

    Holo-(acyl carrier protein) synthase (AcpS), a member of the phosphopantetheinyl transferase superfamily, plays a crucial role in the functional activation of acyl carrier protein (ACP) in the fatty acid biosynthesis pathway. AcpS catalyzes the attachment of the 4'-phosphopantetheinyl moiety of coenzyme A (CoA) to the sidechain of a conserved serine residue on apo-ACP.


  • Organizational Affiliation

    Biological Chemistry, Wyeth-Ayerst Research, Cambridge, MA 02140, USA. parrisk@war.wyeth.com


Macromolecules
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
HOLO-(ACYL CARRIER PROTEIN) SYNTHASE
A, B, C, D, E
A, B, C, D, E, F
122Bacillus subtilisMutation(s): 1 
EC: 2.7.8.7
UniProt
Find proteins for P96618 (Bacillus subtilis (strain 168))
Explore P96618 
Go to UniProtKB:  P96618
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupP96618
Sequence Annotations
Expand
  • Reference Sequence
Small Molecules
Ligands 4 Unique
IDChains Name / Formula / InChI Key2D Diagram3D Interactions
DTT
Query on DTT

Download Ideal Coordinates CCD File 
FA [auth D],
GA [auth D],
M [auth A],
NA [auth F],
Y [auth C]
2,3-DIHYDROXY-1,4-DITHIOBUTANE
C4 H10 O2 S2
VHJLVAABSRFDPM-IMJSIDKUSA-N
GOL
Query on GOL

Download Ideal Coordinates CCD File 
HA [auth D],
R [auth B]
GLYCEROL
C3 H8 O3
PEDCQBHIVMGVHV-UHFFFAOYSA-N
CL
Query on CL

Download Ideal Coordinates CCD File 
BA [auth D]
CA [auth D]
DA [auth D]
EA [auth D]
I [auth A]
BA [auth D],
CA [auth D],
DA [auth D],
EA [auth D],
I [auth A],
J [auth A],
JA [auth E],
K [auth A],
KA [auth F],
L [auth A],
LA [auth F],
MA [auth F],
P [auth B],
Q [auth B],
T [auth C],
U [auth C],
V [auth C],
W [auth C],
X [auth C]
CHLORIDE ION
Cl
VEXZGXHMUGYJMC-UHFFFAOYSA-M
NA
Query on NA

Download Ideal Coordinates CCD File 
AA [auth D]
G [auth A]
H [auth A]
IA [auth E]
N [auth B]
AA [auth D],
G [auth A],
H [auth A],
IA [auth E],
N [auth B],
O [auth B],
S [auth C],
Z [auth D]
SODIUM ION
Na
FKNQFGJONOIPTF-UHFFFAOYSA-N
Experimental Data & Validation

Experimental Data

  • Method: X-RAY DIFFRACTION
  • Resolution: 1.80 Å
  • R-Value Free: 0.219 
  • R-Value Work: 0.196 
  • R-Value Observed: 0.197 
  • Space Group: P 1 21 1
Unit Cell:
Length ( Å )Angle ( ˚ )
a = 76.262α = 90
b = 76.16β = 93.32
c = 85.693γ = 90
Software Package:
Software NamePurpose
SHARPphasing
CNSrefinement
DENZOdata reduction
SCALEPACKdata scaling

Structure Validation

View Full Validation Report



Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 2001-06-27
    Type: Initial release
  • Version 1.1: 2008-04-27
    Changes: Version format compliance
  • Version 1.2: 2011-07-13
    Changes: Non-polymer description, Version format compliance
  • Version 1.3: 2018-01-31
    Changes: Experimental preparation
  • Version 1.4: 2021-11-03
    Changes: Database references, Derived calculations
  • Version 1.5: 2024-02-07
    Changes: Data collection