1F0C

STRUCTURE OF THE VIRAL SERPIN CRMA

PDB DOI: https://doi.org/10.2210/pdb1F0C/pdb

Classification: VIRAL PROTEIN
Organism(s): Cowpox virus
Expression System: Escherichia coli BL21(DE3)
Mutation(s): No

Deposited: 2000-05-15 Released: 2000-09-06
Deposition Author(s): Renatus, M., Zhou, Q., Stennicke, H.R., Snipas, S.J., Turk, D., Bankston, L.A., Liddington, R.C., Salvesen, G.S.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.26 Å
R-Value Free: 0.272
R-Value Work: 0.228

wwPDB Validation 3D Report Full Report

This is version 1.4 of the entry. See complete history.

Literature

Crystal structure of the apoptotic suppressor CrmA in its cleaved form.

Renatus, M., Zhou, Q., Stennicke, H.R., Snipas, S.J., Turk, D., Bankston, L.A., Liddington, R.C., Salvesen, G.S.

(2000) Structure 8: 789-797

PubMed: 10903953 Search on PubMed
DOI: https://doi.org/10.1016/s0969-2126(00)00165-9
Primary Citation of Related Structures:
1F0C

PubMed Abstract:
Cowpox virus expresses the serpin CrmA (cytokine response modifier A) in order to avoid inflammatory and apoptotic responses of infected host cells. The targets of CrmA are members of the caspase family of proteases that either initiate the extrinsic pathway of apoptosis (caspases 8 and 10) or trigger activation of the pro-inflammatory cytokines interleukin-1beta and interleukin-18 (caspase 1).

Organizational Affiliation

Program in Apoptosis and Cell Death Research, The Burnham Institute, La Jolla, CA 92037, USA.

Macromolecule Content

Total Structure Weight: 38.28 kDa
Atom Count: 2,776
Modelled Residue Count: 333
Deposited Residue Count: 341
Unique protein chains: 2

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
ICE INHIBITOR	A	305	Cowpox virus	Mutation(s): 0
UniProt
Find proteins for P07385 (Cowpox virus (strain Brighton Red)) Explore P07385 Go to UniProtKB: P07385
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	P07385
Sequence Annotations Expand
Reference Sequence

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 2
Molecule	Chains	Sequence Length	Organism	Details	Image
ICE INHIBITOR	B	36	Cowpox virus	Mutation(s): 0
UniProt
Find proteins for P07385 (Cowpox virus (strain Brighton Red)) Explore P07385 Go to UniProtKB: P07385
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	P07385
Sequence Annotations Expand
Reference Sequence

Small Molecules

Ligands 1 Unique
ID	Chains	Name / Formula / InChI Key	2D Diagram	3D Interactions
DTT Query on DTT Download Ideal Coordinates CCD File SDF format, chain C [auth A] MOL2 format, chain C [auth A]	C [auth A]	2,3-DIHYDROXY-1,4-DITHIOBUTANE C₄ H₁₀ O₂ S₂ VHJLVAABSRFDPM-IMJSIDKUSA-N		Interactions Focus chain C [auth A] Interactions & Density Focus chain C [auth A]

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 2.26 Å
R-Value Free: 0.272
R-Value Work: 0.228
Space Group: P 2₁ 2₁ 2₁

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 49.203	α = 90
b = 92.455	β = 90
c = 100.454	γ = 90

Software Package:

Software Name	Purpose
AMoRE	phasing
CNS	refinement
MADNESS	data reduction
SCALEPACK	data scaling

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2000-09-06

Deposition Author(s):

Revision History (Full details and data files)

Version 1.0: 2000-09-06
Type: Initial release
Version 1.1: 2008-04-27
Changes: Version format compliance
Version 1.2: 2011-07-13
Changes: Version format compliance
Version 1.3: 2017-10-04
Changes: Advisory, Refinement description
Version 1.4: 2024-02-07
Changes: Advisory, Data collection, Database references, Derived calculations