Crystal structure of N-carbamyl-D-amino acid amidohydrolase with a novel catalytic framework common to amidohydrolases.
Nakai, T., Hasegawa, T., Yamashita, E., Yamamoto, M., Kumasaka, T., Ueki, T., Nanba, H., Ikenaka, Y., Takahashi, S., Sato, M., Tsukihara, T.(2000) Structure 8: 729-737
- PubMed: 10903946 
- DOI: https://doi.org/10.1016/s0969-2126(00)00160-x
- Primary Citation of Related Structures:  
1ERZ - PubMed Abstract: 
N-carbamyl-D-amino acid amidohydrolase (DCase) catalyzes the hydrolysis of N-carbamyl-D-amino acids to the corresponding D-amino acids, which are useful intermediates in the preparation of beta-lactam antibiotics. To understand the catalytic mechanism of N-carbamyl-D-amino acid hydrolysis, the substrate specificity and thermostability of the enzyme, we have determined the structure of DCase from Agrobacterium sp. strain KNK712.
Organizational Affiliation: 
Kaneka Corporation, Takasago-cho, Takasago, 676-8688, Japan. tnakai@kaneka.co.jp