1EHN
CRYSTAL STRUCTURE OF CHITINASE A MUTANT E315Q COMPLEXED WITH OCTA-N-ACETYLCHITOOCTAOSE (NAG)8.
- PDB DOI: https://doi.org/10.2210/pdb1EHN/pdb
- Classification: HYDROLASE
- Organism(s): Serratia marcescens
- Expression System: Escherichia coli
- Mutation(s): Yes 
- Deposited: 2000-02-22 Released: 2001-02-22 
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.90 Å
- R-Value Free: 0.217 
- R-Value Work: 0.173 
- R-Value Observed: 0.175 
wwPDB Validation   3D Report Full Report
This is version 2.2 of the entry. See complete history. 
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
---|---|---|---|---|---|
Molecule | Chains | Sequence Length | Organism | Details | Image |
CHITINASE A | 540 | Serratia marcescens | Mutation(s): 1  EC: 3.2.1.14 | ||
UniProt | |||||
Find proteins for P07254 (Serratia marcescens) Explore P07254  Go to UniProtKB:  P07254 | |||||
Entity Groups   | |||||
Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
UniProt Group | P07254 | ||||
Sequence AnnotationsExpand | |||||
|
Oligosaccharides
Entity ID: 2 | |||||
---|---|---|---|---|---|
Molecule | Chains | Length | 2D Diagram | Glycosylation | 3D Interactions |
2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | B | 8 | N/A | ||
Glycosylation Resources | |||||
GlyTouCan:  G83257UC GlyCosmos:  G83257UC |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.90 Å
- R-Value Free: 0.217 
- R-Value Work: 0.173 
- R-Value Observed: 0.175 
- Space Group: C 2 2 21
Unit Cell:
Length ( Å ) | Angle ( ˚ ) |
---|---|
a = 200.44 | α = 90 |
b = 131.617 | β = 90 |
c = 59.344 | γ = 90 |
Software Name | Purpose |
---|---|
DENZO | data reduction |
SCALEPACK | data scaling |
AMoRE | phasing |
REFMAC | refinement |
Entry History 
Deposition Data
- Released Date: 2001-02-22  Deposition Author(s): Papanikolau, Y., Prag, G., Tavlas, G., Vorgias, C.E., Oppenheim, A.B., Petratos, K.
Revision History (Full details and data files)
- Version 1.0: 2001-02-22
Type: Initial release - Version 1.1: 2008-04-27
Changes: Version format compliance - Version 1.2: 2011-07-13
Changes: Non-polymer description, Version format compliance - Version 2.0: 2020-07-29
Type: Remediation
Reason: Carbohydrate remediation
Changes: Atomic model, Data collection, Derived calculations, Structure summary - Version 2.1: 2021-11-03
Changes: Database references, Structure summary - Version 2.2: 2023-08-09
Changes: Data collection, Refinement description