1E3U

MAD structure of OXA10 class D beta-lactamase

PDB DOI: https://doi.org/10.2210/pdb1E3U/pdb

Classification: BETA-LACTAMASE
Organism(s): Pseudomonas aeruginosa
Expression System: Escherichia coli BL21(DE3)
Mutation(s): No

Deposited: 2000-06-23 Released: 2001-01-12
Deposition Author(s): Maveyraud, L., Golemi, D., Kotra, L.P., Tranier, S., Vakulenko, S., Mobashery, S., Samama, J.P.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 1.66 Å
R-Value Free: 0.210
R-Value Work: 0.181

wwPDB Validation 3D Report Full Report

This is version 1.4 of the entry. See complete history.

Literature

Insights Into Class D Beta-Lactamases are Revealed by the Crystal Structure of the Oxa10 Enzyme from Pseudomonas Aeruginosa

Maveyraud, L., Golemi, D., Kotra, L.P., Tranier, S., Vakulenko, S., Mobashery, S., Samama, J.P.

(2000) Structure 8: 1289

PubMed: 11188693 Search on PubMed
DOI: https://doi.org/10.1016/s0969-2126(00)00534-7
Primary Citation of Related Structures:
1E3U, 1E4D

PubMed Abstract:
beta-lactam antibiotic therapies are commonly challenged by the hydrolytic activities of beta-lactamases in bacteria. These enzymes have been grouped into four classes: A, B, C, and D. Class B beta-lactamases are zinc dependent, and enzymes of classes A, C, and D are transiently acylated on a serine residue in the course of the turnover chemistry. While class A and C beta-lactamases have been extensively characterized by biochemical and structural methods, class D enzymes remain the least studied despite their increasing importance in the clinic.

Organizational Affiliation

Groupe de Cristallographie Biologique IPBS-CNRS, Toulouse, France.

Macromolecule Content

Total Structure Weight: 114.26 kDa
Atom Count: 8,833
Modelled Residue Count: 970
Deposited Residue Count: 984
Unique protein chains: 2

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
BETA-LACTAMASE OXA-10	A, B, C	246	Pseudomonas aeruginosa	Mutation(s): 0 EC: 3.5.2.6
UniProt
Find proteins for P14489 (Pseudomonas aeruginosa) Explore P14489 Go to UniProtKB: P14489
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	P14489
Sequence Annotations Expand
Reference Sequence

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 2
Molecule	Chains	Sequence Length	Organism	Details	Image
BETA-LACTAMASE OXA-10	D	246	Pseudomonas aeruginosa	Mutation(s): 0 EC: 3.5.2.6
UniProt
Find proteins for P14489 (Pseudomonas aeruginosa) Explore P14489 Go to UniProtKB: P14489
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	P14489
Sequence Annotations Expand
Reference Sequence

Small Molecules

Ligands 3 Unique
ID	Chains	Name / Formula / InChI Key	2D Diagram	3D Interactions
AUC Query on AUC Download Ideal Coordinates CCD File SDF format, chain N [auth B] SDF format, chain AA [auth C] SDF format, chain E [auth A] SDF format, chain GA [auth D] SDF format, chain HA [auth D] SDF format, chain IA [auth D] SDF format, chain Y [auth C] SDF format, chain Z [auth C] MOL2 format, chain N [auth B] MOL2 format, chain AA [auth C] MOL2 format, chain E [auth A] MOL2 format, chain GA [auth D] MOL2 format, chain HA [auth D] MOL2 format, chain IA [auth D] MOL2 format, chain Y [auth C] MOL2 format, chain Z [auth C]	AA [auth C] E [auth A] GA [auth D] HA [auth D] IA [auth D] AA [auth C], E [auth A], GA [auth D], HA [auth D], IA [auth D], N [auth B], Y [auth C], Z [auth C]	GOLD (I) CYANIDE ION C₂ Au N₂ DGOHFTDNMSZWDQ-UHFFFAOYSA-N		Interactions Focus chain AA [auth C] Focus chain E [auth A] Focus chain GA [auth D] Focus chain HA [auth D] Focus chain IA [auth D] Focus chain N [auth B] Focus chain Y [auth C] Focus chain Z [auth C] Interactions & Density Focus chain AA [auth C] Focus chain E [auth A] Focus chain GA [auth D] Focus chain HA [auth D] Focus chain IA [auth D] Focus chain N [auth B] Focus chain Y [auth C] Focus chain Z [auth C]
SO4 Query on SO4 Download Ideal Coordinates CCD File SDF format, chain O [auth B] SDF format, chain Q [auth B] SDF format, chain BA [auth C] SDF format, chain CA [auth C] SDF format, chain F [auth A] SDF format, chain G [auth A] SDF format, chain H [auth A] SDF format, chain I [auth A] SDF format, chain J [auth A] SDF format, chain JA [auth D] SDF format, chain K [auth A] SDF format, chain KA [auth D] SDF format, chain LA [auth D] SDF format, chain P [auth B] SDF format, chain R [auth B] MOL2 format, chain O [auth B] MOL2 format, chain Q [auth B] MOL2 format, chain BA [auth C] MOL2 format, chain CA [auth C] MOL2 format, chain F [auth A] MOL2 format, chain G [auth A] MOL2 format, chain H [auth A] MOL2 format, chain I [auth A] MOL2 format, chain J [auth A] MOL2 format, chain JA [auth D] MOL2 format, chain K [auth A] MOL2 format, chain KA [auth D] MOL2 format, chain LA [auth D] MOL2 format, chain P [auth B] MOL2 format, chain R [auth B]	BA [auth C] CA [auth C] F [auth A] G [auth A] H [auth A] BA [auth C], CA [auth C], F [auth A], G [auth A], H [auth A], I [auth A], J [auth A], JA [auth D], K [auth A], KA [auth D], LA [auth D], O [auth B], P [auth B], Q [auth B], R [auth B]	SULFATE ION O₄ S QAOWNCQODCNURD-UHFFFAOYSA-L		Interactions Focus chain BA [auth C] Focus chain CA [auth C] Focus chain F [auth A] Focus chain G [auth A] Focus chain H [auth A] Focus chain I [auth A] Focus chain J [auth A] Focus chain JA [auth D] Focus chain K [auth A] Focus chain KA [auth D] Focus chain LA [auth D] Focus chain O [auth B] Focus chain P [auth B] Focus chain Q [auth B] Focus chain R [auth B] Interactions & Density Focus chain BA [auth C] Focus chain CA [auth C] Focus chain F [auth A] Focus chain G [auth A] Focus chain H [auth A] Focus chain I [auth A] Focus chain J [auth A] Focus chain JA [auth D] Focus chain K [auth A] Focus chain KA [auth D] Focus chain LA [auth D] Focus chain O [auth B] Focus chain P [auth B] Focus chain Q [auth B] Focus chain R [auth B]
EDO Query on EDO Download Ideal Coordinates CCD File SDF format, chain FA [auth C] SDF format, chain U [auth B] SDF format, chain V [auth B] SDF format, chain W [auth B] SDF format, chain DA [auth C] SDF format, chain EA [auth C] SDF format, chain L [auth A] SDF format, chain M [auth A] SDF format, chain MA [auth D] SDF format, chain S [auth B] SDF format, chain T [auth B] SDF format, chain X [auth B] MOL2 format, chain FA [auth C] MOL2 format, chain U [auth B] MOL2 format, chain V [auth B] MOL2 format, chain W [auth B] MOL2 format, chain DA [auth C] MOL2 format, chain EA [auth C] MOL2 format, chain L [auth A] MOL2 format, chain M [auth A] MOL2 format, chain MA [auth D] MOL2 format, chain S [auth B] MOL2 format, chain T [auth B] MOL2 format, chain X [auth B]	DA [auth C] EA [auth C] FA [auth C] L [auth A] M [auth A] DA [auth C], EA [auth C], FA [auth C], L [auth A], M [auth A], MA [auth D], S [auth B], T [auth B], U [auth B], V [auth B], W [auth B], X [auth B]	1,2-ETHANEDIOL C₂ H₆ O₂ LYCAIKOWRPUZTN-UHFFFAOYSA-N		Interactions Focus chain DA [auth C] Focus chain EA [auth C] Focus chain FA [auth C] Focus chain L [auth A] Focus chain M [auth A] Focus chain MA [auth D] Focus chain S [auth B] Focus chain T [auth B] Focus chain U [auth B] Focus chain V [auth B] Focus chain W [auth B] Focus chain X [auth B] Interactions & Density Focus chain DA [auth C] Focus chain EA [auth C] Focus chain FA [auth C] Focus chain L [auth A] Focus chain M [auth A] Focus chain MA [auth D] Focus chain S [auth B] Focus chain T [auth B] Focus chain U [auth B] Focus chain V [auth B] Focus chain W [auth B] Focus chain X [auth B]