1CUK

ESCHERICHIA COLI RUVA PROTEIN AT PH 4.9 AND ROOM TEMPERATURE

PDB DOI: https://doi.org/10.2210/pdb1CUK/pdb

Classification: HELICASE
Organism(s): Escherichia coli
Expression System: Escherichia coli
Mutation(s): No

Deposited: 1996-08-28 Released: 1997-10-22
Deposition Author(s): Rafferty, J.B., Rice, D.W.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 1.90 Å
R-Value Work: 0.209

wwPDB Validation 3D Report Full Report

This is version 1.3 of the entry. See complete history.

Literature

Crystal structure of DNA recombination protein RuvA and a model for its binding to the Holliday junction.

Rafferty, J.B., Sedelnikova, S.E., Hargreaves, D., Artymiuk, P.J., Baker, P.J., Sharples, G.J., Mahdi, A.A., Lloyd, R.G., Rice, D.W.

(1996) Science 274: 415-421

PubMed: 8832889 Search on PubMed
DOI: https://doi.org/10.1126/science.274.5286.415
Primary Citation of Related Structures:
1CUK

PubMed Abstract:
The Escherichia coli DNA binding protein RuvA acts in concert with the helicase RuvB to drive branch migration of Holliday intermediates during recombination and DNA repair. The atomic structure of RuvA was determined at a resolution of 1.9 angstroms. Four monomers of RuvA are related by fourfold symmetry in a manner reminiscent of a four-petaled flower. The four DNA duplex arms of a Holliday junction can be modeled in a square planar configuration and docked into grooves on the concave surface of the protein around a central pin that may facilitate strand separation during the migration reaction. The model presented reveals how a RuvAB-junction complex may also accommodate the resolvase RuvC.

Organizational Affiliation

Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, UK. d.rice@sheffield.ac.uk

Macromolecule Content

Total Structure Weight: 22.2 kDa
Atom Count: 1,428
Modelled Residue Count: 190
Deposited Residue Count: 203
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
RUVA PROTEIN	A	203	Escherichia coli	Mutation(s): 0
UniProt
Find proteins for P0A809 (Escherichia coli (strain K12)) Explore P0A809 Go to UniProtKB: P0A809
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	P0A809
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 1.90 Å
R-Value Work: 0.209
Space Group: P 4

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 83.7	α = 90
b = 83.7	β = 90
c = 33.1	γ = 90

Software Package:

Software Name	Purpose
TNT	refinement
SDMS	data reduction

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 1997-10-22

Deposition Author(s):

Rafferty, J.B.

Rice, D.W.

Revision History (Full details and data files)

Version 1.0: 1997-10-22
Type: Initial release
Version 1.1: 2008-03-24
Changes: Version format compliance
Version 1.2: 2011-07-13
Changes: Derived calculations, Version format compliance
Version 1.3: 2024-02-07
Changes: Data collection, Database references, Other