Download MendeleyStructure solution and refinment of recombinant human salivary amylase
Ramasubbu, N., Sekar, K., Velmurugan, D.To be published.
1C8Q
STRUCTURE SOLUTION AND REFINEMENT OF THE RECOMBINANT HUMAN SALIVARY AMYLASE
- PDB DOI: https://doi.org/10.2210/pdb1C8Q/pdb
- Classification: HYDROLASE
- Organism(s): Homo sapiens
- Expression System: Spodoptera frugiperda
- Mutation(s): No
- Deposited: 2000-06-08 Released: 2001-06-13
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.30 Å
- R-Value Free: 0.216
- R-Value Work: 0.173
wwPDB Validation 3D Report Full Report
This is version 2.1 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| ALPHA-AMYLASE | 496 | Homo sapiens | Mutation(s): 0 EC: 3.2.1.1 |  | |
UniProt & NIH Common Fund Data Resources | |||||
Find proteins for P0DUB6 (Homo sapiens) Explore P0DUB6 Go to UniProtKB: P0DUB6 | |||||
GTEx: ENSG00000237763 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P0DUB6 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| CA Query on CA | B [auth A] | CALCIUM ION Ca BHPQYMZQTOCNFJ-UHFFFAOYSA-N |  | ||
| CL Query on CL | C [auth A] | CHLORIDE ION Cl VEXZGXHMUGYJMC-UHFFFAOYSA-M |  | ||
| Modified Residues 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Type | Formula | 2D Diagram | Parent |
| PCA Query on PCA | A | L-PEPTIDE LINKING | C5 H7 N O3 |  | GLN |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.30 Å
- R-Value Free: 0.216
- R-Value Work: 0.173
- Space Group: P 21 21 21
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 52.707 | α = 90 |
| b = 74.768 | β = 90 |
| c = 133.337 | γ = 90 |
| Software Name | Purpose |
|---|---|
| CNS | refinement |
| CNS | phasing |
Entry History
Deposition Data
- Released Date: 2001-06-13 Deposition Author(s): Ramasubbu, N., Sekar, K., Velmurugan, D.
Revision History (Full details and data files)
- Version 1.0: 2001-06-13
Type: Initial release - Version 1.1: 2008-04-26
Changes: Version format compliance - Version 1.2: 2011-07-13
Changes: Version format compliance - Version 1.3: 2017-10-04
Changes: Refinement description - Version 2.0: 2019-12-25
Changes: Database references, Derived calculations, Polymer sequence - Version 2.1: 2023-08-09
Changes: Data collection, Database references, Derived calculations, Refinement description
