1BCJ
MANNOSE-BINDING PROTEIN-A MUTANT (QPDWGHV) COMPLEXED WITH N-ACETYL-D-GALACTOSAMINE
- PDB DOI: https://doi.org/10.2210/pdb1BCJ/pdb
- Classification: LECTIN
- Organism(s): Rattus norvegicus
- Expression System: Escherichia coli
- Mutation(s): Yes 
- Deposited: 1998-04-30 Released: 1998-06-17 
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.10 Å
- R-Value Free: 0.261 
- R-Value Work: 0.220 
- R-Value Observed: 0.220 
This is version 1.5 of the entry. See complete history. 
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
---|---|---|---|---|---|
Molecule | Chains | Sequence Length | Organism | Details | Image |
MANNOSE-BINDING PROTEIN-A | A [auth 1], B [auth 2], C [auth 3] | 154 | Rattus norvegicus | Mutation(s): 7  | |
UniProt | |||||
Find proteins for P19999 (Rattus norvegicus) Explore P19999  Go to UniProtKB:  P19999 | |||||
Entity Groups   | |||||
Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
UniProt Group | P19999 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
Ligands 3 Unique | |||||
---|---|---|---|---|---|
ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
NGA Query on NGA | D [auth 1], I [auth 2], M [auth 3] | 2-acetamido-2-deoxy-beta-D-galactopyranose C8 H15 N O6 OVRNDRQMDRJTHS-JAJWTYFOSA-N | |||
CA Query on CA | E [auth 1] F [auth 1] G [auth 1] J [auth 2] K [auth 2] | CALCIUM ION Ca BHPQYMZQTOCNFJ-UHFFFAOYSA-N | |||
CL Query on CL | H [auth 1], Q [auth 3] | CHLORIDE ION Cl VEXZGXHMUGYJMC-UHFFFAOYSA-M |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.10 Å
- R-Value Free: 0.261 
- R-Value Work: 0.220 
- R-Value Observed: 0.220 
- Space Group: C 1 2 1
Unit Cell:
Length ( Å ) | Angle ( ˚ ) |
---|---|
a = 80.92 | α = 90 |
b = 84.53 | β = 104.49 |
c = 97.86 | γ = 90 |
Software Name | Purpose |
---|---|
DENZO | data reduction |
SCALEPACK | data scaling |
CNS | refinement |
CNS | phasing |
Entry History 
Deposition Data
- Released Date: 1998-06-17  Deposition Author(s): Kolatkar, A.R., Weis, W.I.
Revision History (Full details and data files)
- Version 1.0: 1998-06-17
Type: Initial release - Version 1.1: 2008-03-03
Changes: Version format compliance - Version 1.2: 2011-07-13
Changes: Version format compliance - Version 1.3: 2020-07-29
Type: Remediation
Reason: Carbohydrate remediation
Changes: Data collection, Database references, Derived calculations, Structure summary - Version 1.4: 2021-11-03
Changes: Database references, Structure summary - Version 1.5: 2023-08-02
Changes: Refinement description