1B8Z

HU FROM THERMOTOGA MARITIMA

PDB DOI: https://doi.org/10.2210/pdb1B8Z/pdb

Classification: DNA BINDING PROTEIN
Organism(s): Thermotoga maritima
Expression System: Escherichia coli
Mutation(s): No

Deposited: 1999-02-03 Released: 2000-02-03
Deposition Author(s): Christodoulou, E., Rypniewski, W.R., Vorgias, C.E.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 1.60 Å
R-Value Free: 0.236
R-Value Work: 0.215

wwPDB Validation 3D Report Full Report

This is version 1.3 of the entry. See complete history.

Literature

Cloning, overproduction, purification and crystallization of the DNA binding protein HU from the hyperthermophilic eubacterium Thermotoga maritima.

Christodoulou, E., Vorgias, C.E.

(1998) Acta Crystallogr D Biol Crystallogr 54: 1043-1045

PubMed: 9757133 Search on PubMed
DOI: https://doi.org/10.1107/s0907444998000341
Primary Citation of Related Structures:
1B8Z

PubMed Abstract:
The humar gene encoding for the histone-like DNA-binding protein HU from the hyperthermophilic eubacterium Thermotoga maritima was efficiently overexpressed in Escherichia coli under the T7 promoter. The HU protein was purified using SP-Sepharose ion-exchange and heparin-affinity chromatography and was successfully crystallized in ammonium sulfate. The crystals were grown in the tetragonal form in space group P43 or P41 and have unit-cell dimensions a = b = 46.12, c = 77.56 A, alpha = beta = gamma = 90 degrees. The crystals diffract X-rays to 1.6 A resolution using synchrotron radiation and are suitable for determination of the HU structure at high resolution.

Organizational Affiliation

European Molecular Biology Laboratory, c/o DESY, Notkestrasse 85, 22603 Hamburg, Germany.

Macromolecule Content

Total Structure Weight: 20.04 kDa
Atom Count: 1,117
Modelled Residue Count: 134
Deposited Residue Count: 180
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
PROTEIN (HISTONELIKE PROTEIN HU)	A, B	90	Thermotoga maritima	Mutation(s): 0
UniProt
Find proteins for P36206 (Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)) Explore P36206 Go to UniProtKB: P36206
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	P36206
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 1.60 Å
R-Value Free: 0.236
R-Value Work: 0.215
Space Group: P 4₃

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 46.12	α = 90
b = 46.12	β = 90
c = 77.56	γ = 90

Software Package:

Software Name	Purpose
AMoRE	phasing
REFMAC	refinement
DENZO	data reduction
SCALEPACK	data scaling

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2000-02-03

Deposition Author(s):

Christodoulou, E.

Rypniewski, W.R.

Revision History (Full details and data files)

Version 1.0: 2000-02-03
Type: Initial release
Version 1.1: 2008-04-26
Changes: Version format compliance
Version 1.2: 2011-07-13
Changes: Version format compliance
Version 1.3: 2023-12-27
Changes: Data collection, Database references

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.