1SFP

CRYSTAL STRUCTURE OF ACIDIC SEMINAL FLUID PROTEIN (ASFP) AT 1.9 A RESOLUTION: A BOVINE POLYPEPTIDE FROM THE SPERMADHESIN FAMILY

PDB DOI: https://doi.org/10.2210/pdb1SFP/pdb

Classification: SPERMADHESIN
Organism(s): Bos taurus
Mutation(s): No

Deposited: 1997-06-24 Released: 1998-06-24
Deposition Author(s): Romao, M.J., Kolln, I., Dias, J.M., Carvalho, A.L., Romero, A., Varela, P.F., Sanz, L., Topfer-Petersen, E., Calvete, J.J.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 1.90 Å
R-Value Observed: 0.173

wwPDB Validation 3D Report Full Report

This is version 1.2 of the entry. See complete history.

Literature

The crystal structures of two spermadhesins reveal the CUB domain fold.

Romero, A., Romao, M.J., Varela, P.F., Kolln, I., Dias, J.M., Carvalho, A.L., Sanz, L., Topfer-Petersen, E., Calvete, J.J.

(1997) Nat Struct Biol 4: 783-788

PubMed: 9334740 Search on PubMed
DOI: https://doi.org/10.1038/nsb1097-783
Primary Citation of Related Structures:
1SFP, 1SPP

PubMed Abstract:
Spermadhesins, 12,000-14,000 M(r) mammalian proteins, include lectins involved in sperm-egg binding and display a single CUB domain architecture. We report the crystal structures of porcine seminal plasma PSP-I/PSP-II, a heterodimer of two glycosylated spermadhesins, and bovine aSFP at 2.4 A and 1.9 A resolution respectively.

Macromolecule Content

Total Structure Weight: 12.93 kDa
Atom Count: 958
Modelled Residue Count: 111
Deposited Residue Count: 114
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
ASFP	A	114	Bos taurus	Mutation(s): 0
UniProt
Find proteins for P29392 (Bos taurus) Explore P29392 Go to UniProtKB: P29392
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	P29392
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 1.90 Å
R-Value Observed: 0.173
Space Group: P 2₁ 2₁ 2

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 41.55	α = 90
b = 52.44	β = 90
c = 48.19	γ = 90

Software Package:

Software Name	Purpose
DENZO	data reduction
SCALEPACK	data scaling
SHELXL-96	model building
SHELXL-96	refinement
SHELXL-96	phasing

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 1998-06-24

Deposition Author(s):

Topfer-Petersen, E.

Revision History (Full details and data files)

Version 1.0: 1998-06-24
Type: Initial release
Version 1.1: 2008-03-24
Changes: Version format compliance
Version 1.2: 2011-07-13
Changes: Version format compliance

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.