1Q8I

Crystal structure of ESCHERICHIA coli DNA Polymerase II

PDB DOI: https://doi.org/10.2210/pdb1Q8I/pdb

Classification: TRANSFERASE
Organism(s): Escherichia coli
Expression System: Escherichia coli
Mutation(s): Yes

Deposited: 2003-08-21 Released: 2004-01-27
Deposition Author(s): Brunzelle, J.S., Muchmore, C.R.A., Mashhoon, N., Blair-Johnson, M., Shuvalova, L., Goodman, M.F., Anderson, W.F., Midwest Center for Structural Genomics (MCSG)

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.00 Å
R-Value Free: 0.236
R-Value Work: 0.197
R-Value Observed: 0.199

wwPDB Validation 3D Report Full Report

This is version 1.4 of the entry. See complete history.

Literature

Crystal Structure of Escherichia Coli DNA Polymerase II

Brunzelle, J.S., Muchmore, C.R.A., Mashhoon, N., Blair-Johnson, M., Shuvalova, L., Goodman, M.F., Anderson, W.F.

To be published.

Macromolecule Content

Total Structure Weight: 90.15 kDa
Atom Count: 6,152
Modelled Residue Count: 708
Deposited Residue Count: 783
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
DNA polymerase II	A	783	Escherichia coli	Mutation(s): 1 Gene Names: POLB OR DINA OR B0060 EC: 2.7.7.7
UniProt
Find proteins for P21189 (Escherichia coli (strain K12)) Explore P21189 Go to UniProtKB: P21189
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	P21189
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 2.00 Å
R-Value Free: 0.236
R-Value Work: 0.197
R-Value Observed: 0.199
Space Group: P 2₁ 2₁ 2

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 92.383	α = 90
b = 116.596	β = 90
c = 82.43	γ = 90

Software Package:

Software Name	Purpose
REFMAC	refinement
DENZO	data reduction
SCALEPACK	data scaling
MLPHARE	phasing

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2004-01-27

Deposition Author(s):

Brunzelle, J.S.

Muchmore, C.R.A.

Blair-Johnson, M.

Midwest Center for Structural Genomics (MCSG)

Revision History (Full details and data files)

Version 1.0: 2004-01-27
Type: Initial release
Version 1.1: 2008-04-29
Changes: Version format compliance
Version 1.2: 2011-07-13
Changes: Advisory, Version format compliance
Version 1.3: 2021-10-27
Changes: Database references
Version 1.4: 2024-02-14
Changes: Data collection

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.