1L6Z

CRYSTAL STRUCTURE OF MURINE CEACAM1A[1,4]: A CORONAVIRUS RECEPTOR AND CELL ADHESION MOLECULE IN THE CEA FAMILY

Experimental Data Snapshot

  • Method: X-RAY DIFFRACTION
  • Resolution: 3.32 Å
  • R-Value Free: 0.329 
  • R-Value Work: 0.295 
  • R-Value Observed: 0.297 

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This is version 2.1 of the entry. See complete history. 


Literature

CRYSTAL STRUCTURE OF MURINE sCEACAM1a[1,4]: A CORONAVIRUS RECEPTOR IN THE CEA FAMILY

Tan, K., Zelus, B.D., Meijers, R., Liu, J.-H., Bergelson, J.M., Duke, N., Zhang, R., Joachimiak, A., Holmes, K.V., Wang, J.-H.

(2002) EMBO J 21: 2076-2086

  • DOI: https://doi.org/10.1093/emboj/21.9.2076
  • Primary Citation of Related Structures:  
    1L6Z

  • PubMed Abstract: 

    CEACAM1 is a member of the carcinoembryonic antigen (CEA) family. Isoforms of murine CEACAM1 serve as receptors for mouse hepatitis virus (MHV), a murine coronavirus. Here we report the crystal structure of soluble murine sCEACAM1a[1,4], which is composed of two Ig-like domains and has MHV neutralizing activity. Its N-terminal domain has a uniquely folded CC' loop that encompasses key virus-binding residues. This is the first atomic structure of any member of the CEA family, and provides a prototypic architecture for functional exploration of CEA family members. We discuss the structural basis of virus receptor activities of murine CEACAM1 proteins, binding of Neisseria to human CEACAM1, and other homophilic and heterophilic interactions of CEA family members.

    • Organizational Affiliation: 

    Dana-Farber Cancer Institute and Department of Medicine, Harvard Medical School, Boston, MA 02115, USA.


Macromolecules
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(by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
biliary glycoprotein C216Mus musculusMutation(s): 0 
Gene Names: Murine CEACAM1A
UniProt
Find proteins for P31809 (Mus musculus)
Explore P31809 
Go to UniProtKB:  P31809
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupP31809
Sequence Annotations
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  • Reference Sequence
Oligosaccharides

Help

Entity ID: 2
MoleculeChains Length2D Diagram Glycosylation3D Interactions
beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose
B
3N-Glycosylation
Glycosylation Resources
GlyTouCan:  G15407YE
GlyCosmos:  G15407YE
GlyGen:  G15407YE
Experimental Data & Validation

Experimental Data

  • Method: X-RAY DIFFRACTION
  • Resolution: 3.32 Å
  • R-Value Free: 0.329 
  • R-Value Work: 0.295 
  • R-Value Observed: 0.297 
  • Space Group: P 31 2 1
Unit Cell:
Length ( Å )Angle ( ˚ )
a = 111.26α = 90
b = 111.26β = 90
c = 65.64γ = 120
Software Package:
Software NamePurpose
HKL-2000data collection
HKL-2000data reduction
MLPHAREphasing
AMoREphasing
X-PLORrefinement
HKL-2000data scaling

Structure Validation

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Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 2002-09-14
    Type: Initial release
  • Version 1.1: 2008-04-28
    Changes: Version format compliance
  • Version 1.2: 2011-07-13
    Changes: Non-polymer description, Version format compliance
  • Version 2.0: 2020-07-29
    Type: Remediation
    Reason: Carbohydrate remediation
    Changes: Advisory, Atomic model, Data collection, Database references, Derived calculations, Non-polymer description, Structure summary
  • Version 2.1: 2023-08-16
    Changes: Data collection, Database references, Refinement description, Structure summary