1K78

Pax5(1-149)+Ets-1(331-440)+DNA


Experimental Data Snapshot

  • Method: X-RAY DIFFRACTION
  • Resolution: 2.25 Å
  • R-Value Free: 0.260 
  • R-Value Work: 0.227 
  • R-Value Observed: 0.231 

wwPDB Validation   3D Report Full Report


This is version 1.3 of the entry. See complete history


Literature

Structural studies of Ets-1/Pax5 complex formation on DNA.

Garvie, C.W.Hagman, J.Wolberger, C.

(2001) Mol Cell 8: 1267-1276

  • DOI: https://doi.org/10.1016/s1097-2765(01)00410-5
  • Primary Citation of Related Structures:  
    1K78, 1K79, 1K7A

  • PubMed Abstract: 

    Pax5 regulates the B cell-specific expression of the mb-1 gene together with members of the Ets family of transcriptional activators. The Ets proteins on their own bind poorly to the Pax5/Ets binding site, but can be recruited to the site by cooperative interactions with Pax5. The structure of the ETS domain of Ets-1 and the paired domain of Pax5 bound to DNA reveals the molecular details of the selective recruitment of different Ets proteins by Pax5. Comparison with structures of Ets-1 alone bound to both high- and low-affinity DNA sites reveals that Pax5 alters the Ets-1 contacts with DNA. The ability of one protein to alter the DNA sequence-specific contacts of another provides a general mechanism for combinatorial regulation of transcription.


  • Organizational Affiliation

    Department of Biophysics and Biophysical Chemistry, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA.


Macromolecules

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Entity ID: 3
MoleculeChains Sequence LengthOrganismDetailsImage
Paired Box Protein Pax5E [auth A],
G [auth E],
I
149Homo sapiensMutation(s): 0 
Gene Names: PAX5
UniProt & NIH Common Fund Data Resources
Find proteins for Q02548 (Homo sapiens)
Explore Q02548 
Go to UniProtKB:  Q02548
PHAROS:  Q02548
GTEx:  ENSG00000196092 
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupQ02548
Sequence Annotations
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  • Reference Sequence
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Entity ID: 4
MoleculeChains Sequence LengthOrganismDetailsImage
C-ets-1 ProteinF [auth B],
H [auth F]
110Mus musculusMutation(s): 0 
Gene Names: ETS-1
UniProt
Find proteins for P27577 (Mus musculus)
Explore P27577 
Go to UniProtKB:  P27577
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupP27577
Sequence Annotations
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  • Reference Sequence
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Entity ID: 1
MoleculeChains LengthOrganismImage
Pax5/Ets Binding Site on the mb-1 promoterA [auth C],
C [auth G]
27N/A
Sequence Annotations
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  • Reference Sequence
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Entity ID: 2
MoleculeChains LengthOrganismImage
Pax5/Ets Binding Site on the mb-1 promoterB [auth D],
D [auth H]
27N/A
Sequence Annotations
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: X-RAY DIFFRACTION
  • Resolution: 2.25 Å
  • R-Value Free: 0.260 
  • R-Value Work: 0.227 
  • R-Value Observed: 0.231 
  • Space Group: P 21 21 21
Unit Cell:
Length ( Å )Angle ( ˚ )
a = 77.84α = 90
b = 89.75β = 90
c = 170.74γ = 90
Software Package:
Software NamePurpose
SOLVEphasing
CNSrefinement
DENZOdata reduction
SCALEPACKdata scaling

Structure Validation

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Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 2002-01-04
    Type: Initial release
  • Version 1.1: 2008-04-27
    Changes: Version format compliance
  • Version 1.2: 2011-07-13
    Changes: Version format compliance
  • Version 1.3: 2024-02-07
    Changes: Data collection, Database references