Download MendeleyCrystal structure of the C-terminal domain of the RAP74 subunit of human transcription factor IIF.
Kamada, K., De Angelis, J., Roeder, R.G., Burley, S.K.(2001) Proc Natl Acad Sci U S A 98: 3115-3120
- PubMed: 11248041
- DOI: https://doi.org/10.1073/pnas.051631098
- Primary Citation of Related Structures:
1I27 - PubMed Abstract:
The x-ray structure of a C-terminal fragment of the RAP74 subunit of human transcription factor (TF) IIF has been determined at 1.02-A resolution. The alpha/beta structure is strikingly similar to the globular domain of linker histone H5 and the DNA-binding domain of hepatocyte nuclear factor 3gamma (HNF-3gamma), making it a winged-helix protein. The surface electrostatic properties of this compact domain differ significantly from those of bona fide winged-helix transcription factors (HNF-3gamma and RFX1) and from the winged-helix domains found within the RAP30 subunit of TFIIF and the beta subunit of TFIIE. RAP74 has been shown to interact with the TFIIF-associated C-terminal domain phosphatase FCP1, and a putative phosphatase binding site has been identified within the RAP74 winged-helix domain.
Organizational Affiliation:
Laboratories of Molecular Biophysics and Biochemistry and Molecular Biology, and Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.
