1EYG

Crystal structure of chymotryptic fragment of E. coli ssb bound to two 35-mer single strand DNAS

PDB DOI: https://doi.org/10.2210/pdb1EYG/pdb
NAKB: 1EYG

Classification: replication/DNA
Organism(s): Escherichia coli
Expression System: Escherichia coli
Mutation(s): No

Deposited: 2000-05-06 Released: 2000-08-01
Deposition Author(s): Raghunathan, S., Waksman, G.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.80 Å
R-Value Free: 0.298
R-Value Work: 0.256
R-Value Observed: 0.256

wwPDB Validation 3D Report Full Report

This is version 1.3 of the entry. See complete history.

Literature

Structure of the DNA binding domain of E. coli SSB bound to ssDNA.

Raghunathan, S., Kozlov, A.G., Lohman, T.M., Waksman, G.

(2000) Nat Struct Biol 7: 648-652

PubMed: 10932248 Search on PubMed
DOI: https://doi.org/10.1038/77943
Primary Citation of Related Structures:
1EYG

PubMed Abstract:
The structure of the homotetrameric DNA binding domain of the single stranded DNA binding protein from Escherichia coli (Eco SSB) bound to two 35-mer single stranded DNAs was determined to a resolution of 2.8 A. This structure describes the vast network of interactions that results in the extensive wrapping of single stranded DNA around the SSB tetramer and suggests a structural basis for its various binding modes.

Organizational Affiliation

Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, Campus Box 8231, 660 S. Euclid Ave., Saint Louis, Missouri 63110, USA.

Macromolecule Content

Total Structure Weight: 71.78 kDa
Atom Count: 4,244
Modelled Residue Count: 491
Deposited Residue Count: 534
Unique protein chains: 1
Unique nucleic acid chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 2
Molecule	Chains	Sequence Length	Organism	Details	Image
SINGLE-STRAND DNA-BINDING PROTEIN	C [auth A], D [auth B], E [auth C], F [auth D]	116	Escherichia coli	Mutation(s): 0
UniProt
Find proteins for P0AGE0 (Escherichia coli (strain K12)) Explore P0AGE0 Go to UniProtKB: P0AGE0
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	P0AGE0
Sequence Annotations Expand
Reference Sequence