Download MendeleyA Quick Solution: Ab Initio Structure Determination of a 19 kDa Metalloproteinase Using Acorn
Mcauley, K.E., Jia-Xing, Y., Dodson, E.J., Lehmbeck, J., Ostergaard, P.R., Wilson, K.S.(2001) Acta Crystallogr D Biol Crystallogr 57: 1571
- PubMed: 11679721
- DOI: https://doi.org/10.1107/s090744490101335x
- Primary Citation of Related Structures:
1EB6 - PubMed Abstract:
A data set from the metalloproteinase deuterolysin was collected at atomic resolution (1.0 A) with synchrotron radiation. The high resolution allowed the structure to be solved with the new direct-methods program ACORN using the coordinates of the Zn atom as a starting point. The phases obtained from ACORN were of sufficient quality to allow automated building to be carried out in ARP/wARP. Minimal manual rebuilding of the model was required and the structure determination was completed using the maximum-likelihood refinement program REFMAC. The whole process, starting from the processed and merged data and ending with a refined model, required less than 6 h of computational time.
Organizational Affiliation:
York Structural Biology Laboratory, Chemistry Department, University of York, Heslington, York YO10 5DD, England.
