Download MendeleyThe Structure and Proposed Catalytic Mechanism of Methionine Gamma-Lyase
Goodall, G., Mottram, J.C., Coombs, G.H., Lapthorn, A.J.To be published.
1E5E
METHIONINE GAMMA-LYASE (MGL) FROM TRICHOMONAS VAGINALIS IN COMPLEX WITH PROPARGYLGLYCINE
- PDB DOI: https://doi.org/10.2210/pdb1E5E/pdb
- Classification: LYASE
- Organism(s): Trichomonas vaginalis G3
- Expression System: Escherichia coli M15
- Mutation(s): No
- Deposited: 2000-07-25 Released: 2001-07-19
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.18 Å
- R-Value Free: 0.193
- R-Value Work: 0.140
wwPDB Validation 3D Report Full Report
This is version 1.3 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| METHIONINE GAMMA-LYASE | 404 | Trichomonas vaginalis G3 | Mutation(s): 0 Gene Names: MGL1 EC: 4.4.1.11 |  | |
UniProt | |||||
Find proteins for A2FEV4 (Trichomonas vaginalis (strain ATCC PRA-98 / G3)) Explore A2FEV4 Go to UniProtKB: A2FEV4 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | A2FEV4 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 3 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| PPJ Query on PPJ | C [auth A], G [auth B] | N-(HYDROXY{3-HYDROXY-2-METHYL-5-[(PHOSPHONOOXY)METHYL]PYRIDIN-4-YL}METHYL)NORVALINE C13 H19 N2 O8 P XZPXXYMXESJHME-PMICCBOBSA-N |  | ||
| SO4 Query on SO4 | D [auth A], E [auth A], H [auth B], I [auth B], J [auth B] | SULFATE ION O4 S QAOWNCQODCNURD-UHFFFAOYSA-L |  | ||
| GOL Query on GOL | F [auth A], K [auth B] | GLYCEROL C3 H8 O3 PEDCQBHIVMGVHV-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.18 Å
- R-Value Free: 0.193
- R-Value Work: 0.140
- Space Group: P 31 1 2
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 88.258 | α = 90 |
| b = 88.258 | β = 90 |
| c = 217.849 | γ = 120 |
| Software Name | Purpose |
|---|---|
| REFMAC | refinement |
| DENZO | data reduction |
| SCALEPACK | data scaling |
| AMoRE | phasing |
Entry History
Deposition Data
- Released Date: 2001-07-19 Deposition Author(s): Goodall, G., Mottram, J.C., Coombs, G.H., Lapthorn, A.J.
Revision History (Full details and data files)
- Version 1.0: 2001-07-19
Type: Initial release - Version 1.1: 2011-11-09
Changes: Atomic model, Derived calculations, Non-polymer description, Other, Structure summary, Version format compliance - Version 1.2: 2014-02-05
Changes: Data collection, Source and taxonomy - Version 1.3: 2023-12-13
Changes: Data collection, Database references, Derived calculations, Refinement description
