Download MendeleyLarge increases in general stabilityfor subtilisin BPN' through incremental changes in the free energy of unfolding
Muralikrishna, P., Wickstrom, E.To be published.
1AQN
SUBTILISIN MUTANT 8324
- PDB DOI: https://doi.org/10.2210/pdb1AQN/pdb
- Classification: SERINE PROTEASE
- Organism(s): Bacillus amyloliquefaciens
- Expression System: Bacillus subtilis
- Mutation(s): Yes
- Deposited: 1997-07-31 Released: 1998-01-14
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.80 Å
wwPDB Validation 3D Report Full Report
This is version 1.3 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| SUBTILISIN 8324 | 275 | Bacillus amyloliquefaciens | Mutation(s): 7 EC: 3.4.21.62 |  | |
UniProt | |||||
Find proteins for P00782 (Bacillus amyloliquefaciens) Explore P00782 Go to UniProtKB: P00782 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P00782 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 3 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| IPA Query on IPA | F [auth A], G [auth A] | ISOPROPYL ALCOHOL C3 H8 O KFZMGEQAYNKOFK-UHFFFAOYSA-N |  | ||
| CA Query on CA | B [auth A], C [auth A] | CALCIUM ION Ca BHPQYMZQTOCNFJ-UHFFFAOYSA-N |  | ||
| UNX Query on UNX | D [auth A], E [auth A] | UNKNOWN ATOM OR ION X |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.80 Å
- Space Group: P 1 21 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 41.64 | α = 90 |
| b = 79.45 | β = 114.53 |
| c = 37.26 | γ = 90 |
| Software Name | Purpose |
|---|---|
| XENGEN | data collection |
| XENGEN | data reduction |
| PROLSQ/PROFFT | model building |
| PROFFT | refinement |
| PROLSQ | refinement |
| XENGEN | data scaling |
Entry History
Deposition Data
- Released Date: 1998-01-14 Deposition Author(s): Whitlow, M., Howard, A.J., Wood, J.F.
Revision History (Full details and data files)
- Version 1.0: 1998-01-14
Type: Initial release - Version 1.1: 2008-03-04
Changes: Version format compliance - Version 1.2: 2011-07-13
Changes: Version format compliance - Version 1.3: 2021-11-03
Changes: Database references, Derived calculations
