Download MendeleyCrystal Structure of Engineered Protein OR494.
Vorobiev, S., Lin, Y.-R., Seetharaman, J., Xiao, R., Everett, J.K., Acton, T.B., Baker, D., Montelione, G.T., Tong, L., Hunt, J.F.To be published.
4PWW
Crystal Structure of Engineered Protein. Northeast Structural Genomics Consortium Target OR494.
- PDB DOI: https://doi.org/10.2210/pdb4PWW/pdb
- Classification: DE NOVO PROTEIN
- Organism(s): synthetic construct
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): No
- Deposited: 2014-03-21 Released: 2014-04-09
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.47 Å
- R-Value Free: 0.216
- R-Value Work: 0.179
- R-Value Observed: 0.181
wwPDB Validation 3D Report Full Report
This is version 1.2 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| OR494 | 89 | synthetic construct | Mutation(s): 0 |  | |
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| PO4 Query on PO4 | B [auth A] | PHOSPHATE ION O4 P NBIIXXVUZAFLBC-UHFFFAOYSA-K |  | ||
| ACY Query on ACY | C [auth A], D [auth A], E [auth A] | ACETIC ACID C2 H4 O2 QTBSBXVTEAMEQO-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.47 Å
- R-Value Free: 0.216
- R-Value Work: 0.179
- R-Value Observed: 0.181
- Space Group: I 21 21 21
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 33.548 | α = 90 |
| b = 69.645 | β = 90 |
| c = 73.138 | γ = 90 |
| Software Name | Purpose |
|---|---|
| PHENIX | refinement |
| PDB_EXTRACT | data extraction |
| HKL-2000 | data collection |
| HKL-2000 | data reduction |
| HKL-2000 | data scaling |
| BALBES | phasing |
Entry History
Deposition Data
- Released Date: 2014-04-09 Deposition Author(s): Vorobiev, S., Lin, Y.-R., Seetharaman, J., Xiao, R., Everett, J.K., Acton, T.B., Baker, D., Montelione, G.T., Tong, L., Hunt, J.F., Northeast Structural Genomics Consortium (NESG)
Revision History (Full details and data files)
- Version 1.0: 2014-04-09
Type: Initial release - Version 1.1: 2017-10-25
Changes: Author supporting evidence - Version 1.2: 2023-09-20
Changes: Data collection, Database references, Derived calculations, Refinement description
