Download MendeleyTurn motif phosphorylation regulates processing of cAMP-dependent protein kinase
Steichen, J.M., Yang, J., Taylor, S.S.To be published.
4DG3
Crystal structure of R336A mutant of cAMP-dependent protein kinase with unphosphorylated turn motif.
- PDB DOI: https://doi.org/10.2210/pdb4DG3/pdb
- Classification: TRANSFERASE/TRANSFERASE INHIBITOR
- Organism(s): Mus musculus
- Expression System: Escherichia coli
- Mutation(s): Yes
- Deposited: 2012-01-24 Released: 2013-02-13
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.80 Å
- R-Value Free: 0.217
- R-Value Work: 0.183
- R-Value Observed: 0.185
This is version 1.0 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| cAMP-dependent protein kinase catalytic subunit alpha | A [auth E] | 371 | Mus musculus | Mutation(s): 1 Gene Names: Prkaca, Pkaca EC: 2.7.11.11 |  |
UniProt | |||||
Find proteins for P05132 (Mus musculus) Explore P05132 Go to UniProtKB: P05132 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P05132 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Find similar proteins by: Sequence | 3D Structure
Entity ID: 2 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| cAMP-dependent protein kinase inhibitor alpha | B [auth A] | 20 | Mus musculus | Mutation(s): 0 |  |
UniProt & NIH Common Fund Data Resources | |||||
Find proteins for P63248 (Mus musculus) Explore P63248 Go to UniProtKB: P63248 | |||||
IMPC: MGI:104747 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P63248 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| ANP Query on ANP | C [auth E] | PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER C10 H17 N6 O12 P3 PVKSNHVPLWYQGJ-KQYNXXCUSA-N |  | ||
| MG Query on MG | D [auth E], E | MAGNESIUM ION Mg JLVVSXFLKOJNIY-UHFFFAOYSA-N |  | ||
| Modified Residues 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Type | Formula | 2D Diagram | Parent |
| SEP Query on SEP | A [auth E] | L-PEPTIDE LINKING | C3 H8 N O6 P |  | SER |
| TPO Query on TPO | A [auth E] | L-PEPTIDE LINKING | C4 H10 N O6 P |  | THR |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.80 Å
- R-Value Free: 0.217
- R-Value Work: 0.183
- R-Value Observed: 0.185
- Space Group: P 21 21 21
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 72.29 | α = 90 |
| b = 76.69 | β = 90 |
| c = 80.31 | γ = 90 |
| Software Name | Purpose |
|---|---|
| ADSC | data collection |
| PHASER | phasing |
| REFMAC | refinement |
| MOSFLM | data reduction |
| SCALA | data scaling |
Entry History
Deposition Data
- Released Date: 2013-02-13 Deposition Author(s): Steichen, J.M., Yang, J., Taylor, S.S.
Revision History (Full details and data files)
- Version 1.0: 2013-02-13
Type: Initial release
