3OF0

crystal structure of the L317I mutant of the chicken c-Src tyrosine kinase domain

PDB DOI: https://doi.org/10.2210/pdb3OF0/pdb

Classification: TRANSFERASE
Organism(s): Gallus gallus
Expression System: Escherichia coli
Mutation(s): Yes

Deposited: 2010-08-13 Released: 2011-08-17
Deposition Author(s): Boubeva, R., Pernot, L., Perozzo, R., Scapozza, L.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.70 Å
R-Value Free: 0.262
R-Value Work: 0.233
R-Value Observed: 0.235

wwPDB Validation 3D Report Full Report

This is version 1.1 of the entry. See complete history.

Literature

a single amino-acid dictates the dynamics of the switch between active and inactive C-Src conformation

Boubeva, R., Pernot, L., Cristiani, A., Moretti, L., Berteotti, A., Perozzo, R., Gervasio, F., Scapozza, L.

To be published.

Macromolecule Content

Total Structure Weight: 65.45 kDa
Atom Count: 4,297
Modelled Residue Count: 511
Deposited Residue Count: 572
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Proto-oncogene tyrosine-protein kinase Src	A, B	286	Gallus gallus	Mutation(s): 1 Gene Names: SRC EC: 2.7.10.2
UniProt
Find proteins for P00523 (Gallus gallus) Explore P00523 Go to UniProtKB: P00523
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	P00523
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 2.70 Å
R-Value Free: 0.262
R-Value Work: 0.233
R-Value Observed: 0.235
Space Group: P 1

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 42.072	α = 78.66
b = 63.496	β = 89.41
c = 74.126	γ = 90

Software Package:

Software Name	Purpose
PHASER	phasing
PHENIX	refinement
MOSFLM	data reduction
SCALA	data scaling

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2011-08-17

Deposition Author(s):

Revision History (Full details and data files)

Version 1.0: 2011-08-17
Type: Initial release
Version 1.1: 2023-09-06
Changes: Data collection, Database references, Refinement description

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.