3E4U

Crystal Structure of the Wild-Type Human BCL6 BTB/POZ Domain

PDB DOI: https://doi.org/10.2210/pdb3E4U/pdb

Classification: TRANSCRIPTION
Organism(s): Homo sapiens
Expression System: Escherichia coli
Mutation(s): No

Deposited: 2008-08-12 Released: 2008-12-09
Deposition Author(s): Stead, M.A., Rosbrook, G.O., Hadden, J.M., Trinh, C.H., Carr, S.B., Wright, S.C.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.10 Å
R-Value Free: 0.259
R-Value Work: 0.219
R-Value Observed: 0.221

wwPDB Validation 3D Report Full Report

This is version 1.3 of the entry. See complete history.

Literature

Structure of the wild-type human BCL6 POZ domain.

Stead, M.A., Rosbrook, G.O., Hadden, J.M., Trinh, C.H., Carr, S.B., Wright, S.C.

(2008) Acta Crystallogr Sect F Struct Biol Cryst Commun 64: 1101-1104

PubMed: 19052359 Search on PubMedSearch on PubMed Central
DOI: https://doi.org/10.1107/S1744309108036063
Primary Citation of Related Structures:
3E4U

PubMed Abstract:
BCL6 is a transcriptional repressor that is overexpressed in diffuse large B-cell lymphoma and follicular lymphoma. The N-terminal POZ domain of BCL6 interacts with transcriptional corepressors and targeting these associations is a promising therapeutic strategy. Previous structural studies of the BCL6 POZ domain have used a mutant form because of the low solubility of the wild-type recombinant protein. A method for the purification and crystallization of the wild-type BCL6 POZ domain is described and the crystal structure to 2.1 A resolution is reported. This will be relevant for the design of therapeutics that target BCL6 POZ-domain interaction interfaces.

Organizational Affiliation

Molecular Cell Biology Research Group, Garstang/Astbury Buildings, Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, England.

Macromolecule Content

Total Structure Weight: 88.42 kDa
Atom Count: 5,827
Modelled Residue Count: 698
Deposited Residue Count: 780
Unique protein chains: 1

Macromolecules

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(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
B-cell lymphoma 6 protein	A, B, C, D, E A, B, C, D, E, F	130	Homo sapiens	Mutation(s): 0 Gene Names: BCL6, BCL5, LAZ3, ZBTB27, ZNF51
UniProt & NIH Common Fund Data Resources
Find proteins for P41182 (Homo sapiens) Explore P41182 Go to UniProtKB: P41182
PHAROS: P41182 GTEx: ENSG00000113916
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	P41182
Sequence Annotations Expand
Reference Sequence