2N4C

EC-NMR Structure of Agrobacterium tumefaciens Atu1203 Determined by Combining Evolutionary Couplings (EC) and Sparse NMR Data. Northeast Structural Genomics Consortium target AtT10


Experimental Data Snapshot

  • Method: SOLUTION NMR
  • Conformers Calculated: 100 
  • Conformers Submitted: 20 
  • Selection Criteria: structures with the lowest energy 

wwPDB Validation   3D Report Full Report


This is version 1.2 of the entry. See complete history

Re-refinement Note

This entry reflects an alternative modeling of the original data in: 2K2P


Literature

Protein structure determination by combining sparse NMR data with evolutionary couplings.

Tang, Y.Huang, Y.J.Hopf, T.A.Sander, C.Marks, D.S.Montelione, G.T.

(2015) Nat Methods 12: 751-754

  • DOI: https://doi.org/10.1038/nmeth.3455
  • Primary Citation of Related Structures:  
    2N42, 2N44, 2N45, 2N46, 2N47, 2N48, 2N49, 2N4A, 2N4B, 2N4C, 2N4D, 2N4F

  • PubMed Abstract: 

    Accurate determination of protein structure by NMR spectroscopy is challenging for larger proteins, for which experimental data are often incomplete and ambiguous. Evolutionary sequence information together with advances in maximum entropy statistical methods provide a rich complementary source of structural constraints. We have developed a hybrid approach (evolutionary coupling-NMR spectroscopy; EC-NMR) combining sparse NMR data with evolutionary residue-residue couplings and demonstrate accurate structure determination for several proteins 6-41 kDa in size.


  • Organizational Affiliation

    1] Center for Advanced Biotechnology and Medicine, Rutgers, The State University of New Jersey, Piscataway, New Jersey, USA. [2] Department of Molecular Biology and Biochemistry, Rutgers, The State University of New Jersey, Piscataway, New Jersey, USA.


Macromolecules
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
Uncharacterized protein Atu120385Agrobacterium fabrum str. C58Mutation(s): 0 
Gene Names: Atu1203
UniProt
Find proteins for A9CJD6 (Agrobacterium fabrum (strain C58 / ATCC 33970))
Explore A9CJD6 
Go to UniProtKB:  A9CJD6
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupA9CJD6
Sequence Annotations
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: SOLUTION NMR
  • Conformers Calculated: 100 
  • Conformers Submitted: 20 
  • Selection Criteria: structures with the lowest energy 

Structure Validation

View Full Validation Report



Entry History 

Revision History  (Full details and data files)

  • Version 1.0: 2015-07-01
    Type: Initial release
  • Version 1.1: 2015-08-26
    Changes: Database references
  • Version 1.2: 2023-06-14
    Changes: Data collection, Database references, Other