1JW2

SOLUTION STRUCTURE OF HEMOLYSIN EXPRESSION MODULATING PROTEIN Hha FROM ESCHERICHIA COLI. Ontario Centre for Structural Proteomics target EC0308_1_72; Northeast Structural Genomics Target ET88


Experimental Data Snapshot

  • Method: SOLUTION NMR
  • Conformers Calculated: 30 
  • Conformers Submitted: 10 
  • Selection Criteria: structures with the lowest energy 

wwPDB Validation   3D Report Full Report


This is version 1.3 of the entry. See complete history


Literature

An NMR approach to structural proteomics

Yee, A.Chang, X.Pineda-Lucena, A.Wu, B.Semesi, A.Le, B.Ramelot, T.Lee, G.M.Bhattacharyya, S.Gutierrez, P.Denisov, A.Lee, C.H.Cort, J.R.Kozlov, G.Liao, J.Finak, G.Chen, L.Wishart, D.Lee, W.McIntosh, L.P.Gehring, K.Kennedy, M.A.Edwards, A.M.Arrowsmith, C.H.

(2002) Proc Natl Acad Sci U S A 99: 1825-1830

  • DOI: https://doi.org/10.1073/pnas.042684599
  • Primary Citation of Related Structures:  
    1JCU, 1JDQ, 1JE3, 1JRM, 1JW2, 1JW3, 1RYJ, 1RYK

  • PubMed Abstract: 

    The influx of genomic sequence information has led to the concept of structural proteomics, the determination of protein structures on a genome-wide scale. Here we describe an approach to structural proteomics of small proteins using NMR spectroscopy. Over 500 small proteins from several organisms were cloned, expressed, purified, and evaluated by NMR. Although there was variability among proteomes, overall 20% of these proteins were found to be readily amenable to NMR structure determination. NMR sample preparation was centralized in one facility, and a distributive approach was used for NMR data collection and analysis. Twelve structures are reported here as part of this approach, which allowed us to infer putative functions for several conserved hypothetical proteins.


  • Organizational Affiliation

    Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, 101 College Street, Toronto, ON, Canada M5G 1L7.


Macromolecules
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
HEMOLYSIN EXPRESSION MODULATING PROTEIN Hha72Escherichia coliMutation(s): 0 
UniProt
Find proteins for P0ACE3 (Escherichia coli (strain K12))
Explore P0ACE3 
Go to UniProtKB:  P0ACE3
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupP0ACE3
Sequence Annotations
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: SOLUTION NMR
  • Conformers Calculated: 30 
  • Conformers Submitted: 10 
  • Selection Criteria: structures with the lowest energy 

Structure Validation

View Full Validation Report



Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 2002-02-27
    Type: Initial release
  • Version 1.1: 2008-04-27
    Changes: Version format compliance
  • Version 1.2: 2011-07-13
    Changes: Version format compliance
  • Version 1.3: 2022-02-23
    Changes: Database references, Derived calculations