POP-OUT | CLOSE

An Information Portal to 105212 Biological Macromolecular Structures

Crystal structure of E. coli PutA proline dehydrogenase domain (residues 86-669) complexed with L-lactate
Biology and Chemistry Report
1TJ1
  •   Structure Details   Hide

    Structure Keywords

    Keywords OXIDOREDUCTASE
    Text beta/alpha barrel, flavoenzyme, FAD, proline catabolism, L-lactate, OXIDOREDUCTASE

    Polymeric Molecules

    Chain A
    Description Bifunctional putA protein 
    Fragment proline dehydrogenase domain (residues 86-669) 
    Nonstandard Linkage no 
    Nonstandard Monomers no 
    Polymer Type polypeptide(L) 
    Formula Weight 66668.8 
    Source Method genetically manipulated  

    Ligands and Prosthetic Groups

    ID Name Chemical Formula Weight Ligand Structure
    2OP  (2S)-2-HYDROXYPROPANOIC ACID  C3 H6 O3   90.08  View 
    FAD  FLAVIN-ADENINE DINUCLEOTIDE  C27 H33 N9 O15 P2   785.55  View 
     
  •   Protein Details   Hide

    UniProtKB Information

    Chain SWS/UNP ID SWS/UNP Accession(s)
    A PUTA_ECOLI P09546     

    Keywords and Names

    Chain(s) RCSB Name UniProtKB Name UniProtKB Keywords

    EC, Associated Pathways and Catalytic Sites

    Chain(s) IUBMB KEGG BioCyc
    1.5.5.2      
    C: Cellular Location | F: Molecular Function | P: Biological Process
    Chain A
    GO ID   Ontology GO Term Definition
    55114   Oxidation Reduction Process  A Metabolic Process That Results in the Removal or Addition of One or More Electrons to or From a Substance with or Without the Concomitant Removal or Addition of a Proton or Protons. 
    3842   1 Pyrroline 5 Carboxylate Dehydrogenase Activity  Catalysis of the Reaction: 1 Pyrroline 5 Carboxylate + Nad+ + H2o = L Glutamate + Nadh + H(+). 


     
  •   Gene Details   Hide

    Genetic Source

    Chain A
    Scientific Name Escherichia coli  
    Genus Escherichia
    Gene puta, poaa, b1014
    Host Scientific Name Escherichia coli  
    Host Genus Escherichia
    Host Strain Bl21 De3 Plyss
    Host Vector Type Plasmid
    Host Plasmid Name pET-23b