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An Information Portal to 105025 Biological Macromolecular Structures

Structure of mammalian cytochrome P450 2B4 with bound 4-(4-chlorophenyl)imidazole
Biology and Chemistry Report
1SUO
  •   Structure Details   Hide

    Structure Keywords

    Keywords OXIDOREDUCTASE
    Text oxidoreductase, membrane protein, CYP 2B4, CYP LM2, Cytochrome p450, Monooxygenase

    Polymeric Molecules

    Chain A
    Description Cytochrome P450 2B4 
    Mutation E2A, G22K, H23K, P24T, K25S, A26S, H27K, R29K, P221S, H226Y 
    Nonstandard Linkage no 
    Nonstandard Monomers no 
    Polymer Type polypeptide(L) 
    Formula Weight 54169.8 
    Source Method genetically manipulated  
    Entity Name CYPIIB4, P450-LM2, Isozyme 2, P450 types B0 and B1 

    Ligands and Prosthetic Groups

    ID Name Chemical Formula Weight Ligand Structure
    CPZ  4-(4-CHLOROPHENYL)IMIDAZOLE  C9 H7 Cl N2   178.62  View 
    HEM  PROTOPORPHYRIN IX CONTAINING FE  C34 H32 Fe N4 O4   616.49  View 
     
  •   Protein Details   Hide

    UniProtKB Information

    Chain SWS/UNP ID SWS/UNP Accession(s)
    A CP2B4_RABIT P00177     

    Keywords and Names

    Chain(s) RCSB Name UniProtKB Name UniProtKB Keywords

    EC, Associated Pathways and Catalytic Sites

    Chain(s) IUBMB KEGG BioCyc Catalytic Site(s)
    1.14.14.1        
    C: Cellular Location | F: Molecular Function | P: Biological Process
    Chain A
    GO ID   Ontology GO Term Definition
    55114   Oxidation Reduction Process  A Metabolic Process That Results in the Removal or Addition of One or More Electrons to or From a Substance with or Without the Concomitant Removal or Addition of a Proton or Protons. 
    5783   Endoplasmic Reticulum  The Irregular Network of Unit Membranes Visible Only by Electron Microscopy That Occurs in the Cytoplasm of Many Eukaryotic Cells. the Membranes Form a Complex Meshwork of Tubular Channels Which Are Often Expanded Into Slitlike Cavities Called Cisternae. the Er Takes Two Forms Rough (or Granular) with Ribosomes Adhering to the Outer Surface and Smooth (with No Ribosomes Attached). 
    5789   Endoplasmic Reticulum Membrane  The Lipid Bilayer Surrounding the Endoplasmic Reticulum. 
    16020   Membrane  Double Layer of Lipid Molecules That Encloses All Cells and in Eukaryotes Many Organelles; May Be a Single or Double Lipid Bilayer; Also Includes Associated Proteins. 
    31090   Organelle Membrane  A Membrane That Is One of the Two Lipid Bilayers of an Organelle Envelope or the Outermost Membrane of Single Membrane Bound Organelle. 
    43231   Intracellular Membrane Bounded Organelle  Organized Structure of Distinctive Morphology and Function Bounded by a Single or Double Lipid Bilayer Membrane and Occurring Within the Cell. Includes the Nucleus Mitochondria Plastids Vacuoles and Vesicles. Excludes the Plasma Membrane. 
    4497   Monooxygenase Activity  Catalysis of the Incorporation of One Atom From Molecular Oxygen Into a Compound and the Reduction of the Other Atom of Oxygen to Water. 
    5506   Iron Ion Binding  Interacting Selectively and Non Covalently with Iron (fe) Ions. 
    16491   Oxidoreductase Activity  Catalysis of an Oxidation Reduction (redox) Reaction a Reversible Chemical Reaction in Which the Oxidation State of an Atom or Atoms Within a Molecule Is Altered. One Substrate Acts As a Hydrogen or Electron Donor and Becomes Oxidized While the Other Acts As Hydrogen or Electron Acceptor and Becomes Reduced. 
    16705   Oxidoreductase Activity Acting On Paired Donors with Incorporation or Reduction of Molecular Oxygen  Catalysis of an Oxidation Reduction (redox) Reaction in Which Hydrogen or Electrons Are Transferred From Each of Two Donors and Molecular Oxygen Is Reduced or Incorporated Into a Donor. 
    16712   Oxidoreductase Activity Acting On Paired Donors with Incorporation or Reduction of Molecular Oxygen Reduced Flavin or Flavoprotein As One Donor and Incorporation of One Atom of Oxygen  Catalysis of an Oxidation Reduction (redox) Reaction in Which Hydrogen or Electrons Are Transferred From Reduced Flavin or Flavoprotein and One Other Donor and One Atom of Oxygen Is Incorporated Into One Donor. 
    20037   Heme Binding  Interacting Selectively and Non Covalently with Heme Any Compound of Iron Complexed in a Porphyrin (tetrapyrrole) Ring. 
    46872   Metal Ion Binding  Interacting Selectively and Non Covalently with Any Metal Ion. 
    70330   Aromatase Activity  Catalysis of the Reduction of an Aliphatic Ring to Yield an Aromatic Ring. 


    Protein Analyses

    Measured Binding Affinities   

     
  •   Gene Details   Hide

    Genetic Source

    Chain A
    Common Name Rabbit
    Scientific Name Oryctolagus cuniculus  
    Genus Oryctolagus
    Gene cyp2b4
    Host Scientific Name Escherichia coli  
    Host Genus Escherichia
    Host Strain Topp3
    Host Vector Type Plasmid
    Host Plasmid Name PKK


    Genome Information

    Chromosome Locus Gene ID Gene Name Symbol
    Un - 100327257     cytochrome P450, family 2, subfamily b, polypeptide 4 CYP2B4