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E. COLI THYMIDYLATE SYNTHASE IN COMPLEX WITH METHOTREXATE (MTX) AND 2'-DEOXYURIDINE 5'-MONOPHOSPHATE (DUMP)
Biology and Chemistry Report
1AXW
  •   Structure Details   Hide

    Structure Keywords

    Keywords METHYLTRANSFERASE
    Text METHYLTRANSFERASE, TRANSFERASE, INHIBITOR

    Polymeric Molecules

    Chain A,B
    Description THYMIDYLATE SYNTHASE 
    Nonstandard Linkage no 
    Nonstandard Monomers yes 
    Polymer Type polypeptide(L) 
    Formula Weight 30543.9 
    Source Method genetically manipulated  
    Entity Name TS, THYMIDYLATE SYNTHETASE 

    Ligands and Prosthetic Groups

    ID Name Chemical Formula Weight Ligand Structure
    MTX  METHOTREXATE  C20 H22 N8 O5   454.44  View 
    UMP  2'-DEOXYURIDINE 5'-MONOPHOSPHATE  C9 H13 N2 O8 P   308.18  View 
     
  •   Protein Details   Hide

    UniProtKB Information

    Chain SWS/UNP ID SWS/UNP Accession(s)
    A,B TYSY_ECOLI P00470     

    Keywords and Names

    Chain(s) RCSB Name UniProtKB Name UniProtKB Keywords

    EC, Associated Pathways and Catalytic Sites

    Chain(s) IUBMB KEGG BioCyc Catalytic Site(s)
    A B  2.1.1.45        
    C: Cellular Location | F: Molecular Function | P: Biological Process
    Chain A,B
    GO ID   Ontology GO Term Definition
    6231   Dtmp Biosynthetic Process  The Chemical Reactions and Pathways Resulting in the Formation of Dtmp Deoxyribosylthymine Monophosphate (2' Deoxyribosylthymine 5' Phosphate). 
    6235   Dttp Biosynthetic Process  The Chemical Reactions and Pathways Resulting in the Formation of Dttp Deoxyribosylthymine Triphosphate. 
    6417   Regulation of Translation  Any Process That Modulates the Frequency Rate or Extent of the Chemical Reactions and Pathways Resulting in the Formation of Proteins by the Translation of Mrna. 
    9165   Nucleotide Biosynthetic Process  The Chemical Reactions and Pathways Resulting in the Formation of Nucleotides Any Nucleoside That Is Esterified with (ortho)phosphate or an Oligophosphate At Any Hydroxyl Group On the Glycose Moiety; May Be Mono Di or Triphosphate; This Definition Includes Cyclic Nucleotides (nucleoside Cyclic Phosphates). 
    32259   Methylation  The Process in Which a Methyl Group Is Covalently Attached to a Molecule. 
    5737   Cytoplasm  All of the Contents of a Cell Excluding the Plasma Membrane and Nucleus But Including Other Subcellular Structures. 
    3723   RNA Binding  Interacting Selectively and Non Covalently with an RNA Molecule or a Portion Thereof. 
    4799   Thymidylate Synthase Activity  Catalysis of the Reaction: 5 10 Methylenetetrahydrofolate + Dump = 7 8 Dihydrofolate + Thymidylate. 
    8168   Methyltransferase Activity  Catalysis of the Transfer of a Methyl Group to an Acceptor Molecule. 
    16740   Transferase Activity  Catalysis of the Transfer of a Group E.g. a Methyl Group Glycosyl Group Acyl Group Phosphorus Containing or Other Groups From One Compound (generally Regarded As the Donor) to Another Compound (generally Regarded As the Acceptor). Transferase Is the Systematic Name For Any Enzyme of EC Class 2. 


    Protein Analyses

    Measured Binding Affinities   

     
  •   Gene Details   Hide

    Genetic Source

    Chain A,B
    Scientific Name Escherichia coli  
    Genus Escherichia
    Cell Line X2913
    Gene thya
    Host Scientific Name Escherichia coli  
    Host Genus Escherichia
    Host Gene Thya
    Host Plasmid Name PTHYA-WT