4G3Y

Crystal structure of TNF-alpha in complex with Infliximab Fab fragment

PDB DOI: https://doi.org/10.2210/pdb4G3Y/pdb

Classification: IMMUNE SYSTEM
Organism(s): Homo sapiens
Expression System: Escherichia coli
Mutation(s): No

Deposited: 2012-07-15 Released: 2013-03-27
Deposition Author(s): Liang, S.Y., Dai, J.X., Guo, Y.J., Lou, Z.Y.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.60 Å
R-Value Free: 0.235
R-Value Work: 0.189
R-Value Observed: 0.191

wwPDB Validation 3D Report Full Report

This is version 1.1 of the entry. See complete history.

Literature

Structural basis for treating tumor necrosis factor alpha (TNFalpha)-associated diseases with the therapeutic antibody infliximab

Liang, S.Y., Dai, J.X., Hou, S., Su, L., Zhang, D., Guo, H., Hu, S., Wang, H., Rao, Z., Guo, Y.J., Lou, Z.Y.

(2013) J Biol Chem 288: 13799-13807

PubMed: 23504311 Search on PubMedSearch on PubMed Central
DOI: https://doi.org/10.1074/jbc.M112.433961
Primary Citation of Related Structures:
4G3Y

PubMed Abstract:
Although infliximab has high efficacy in treating TNFα-associated diseases, the epitope on TNFα remains unclear.

Organizational Affiliation

Laboratory of Structural Biology and Ministry of Education Laboratory of Protein Science, School of Medicine, Tsinghua University, Beijing 100084, China.

Macromolecule Content

Total Structure Weight: 65.19 kDa
Atom Count: 4,599
Modelled Residue Count: 582
Deposited Residue Count: 597
Unique protein chains: 3

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
infliximab Fab L	A [auth L]	214	Homo sapiens	Mutation(s): 0
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
Sequence Annotations Expand
Reference Sequence

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 2
Molecule	Chains	Sequence Length	Organism	Details	Image
infliximab Fab H	B [auth H]	226	Homo sapiens	Mutation(s): 0
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
Sequence Annotations Expand
Reference Sequence

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 3
Molecule	Chains	Sequence Length	Organism	Details	Image
Tumor necrosis factor	C	157	Homo sapiens	Mutation(s): 0 Gene Names: TNFA
UniProt & NIH Common Fund Data Resources
Find proteins for P01375 (Homo sapiens) Explore P01375 Go to UniProtKB: P01375
PHAROS: P01375 GTEx: ENSG00000232810
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	P01375
Sequence Annotations Expand
Reference Sequence