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Crystal structure of RB69 gp43 in complex with DNA with 5-NIMP opposite an abasic site analog
 
 
DOI:10.2210/pdb2oyq/pdb   NDB ID: PD0979
2OYQ
 
Primary Citation
spinning wheel
 
 
  •  
    Move Section Molecular Description Hide
    Classification: Transferase/dna
    Structure Weight: 464590.15
     
    Molecule:Template DNA
    Polymer:1Type:polydeoxyribonucleotideLength:21
    Chains:E, G, I, K
    Molecule:Primer DNA
    Polymer:2Type:polydeoxyribonucleotideLength:15
    Chains:F, H, J, L
    Molecule:DNA polymerase
    Polymer:3Type:polypeptide(L)Length:903
    Chains:A, B, C, D
    EC#:2.7.7.7 Go to IUBMB EC entry  
    Mutation:D222A, D327A
     
     
  •  
    Move Section Source Hide
    Polymer: 1
    Scientific Name: Synthetic construct Go to NCBI Taxonomy entry  
    Polymer: 2
    Scientific Name: Synthetic construct Go to NCBI Taxonomy entry  
    Polymer: 3
    Scientific Name: Enterobacteria phage rb69 Go to NCBI Taxonomy entry Expression System: Escherichia coli bl21(de3)  
     
     
  •  
    Move Section Related PDB Entries Hide
    Id Details
    1IG9   
    1RV2   
    2OZM   
    2OZS   
    2P5G   
     
     
  •  
    Move Section Ligand Chemical Component Hide
    Identifier Name Formula Interaction View Links
    MG     MAGNESIUM ION Mg 3DLigand Explorer Link out to Ligand Expos:MG Link out to SuperLigands:MG Link out to SuperHapten:MG
    N5P     1-{2-DEOXY-5-O-[(R)-HYDROXY{[(R)-HYDROXY(PHOSPHONOOXY)PHOSPHORYL]OXY}PHOSPHORYL]
    - BETA-D-ERYTHRO-PENTOFURANOSYL}-5-NITRO-1H- INDOLE
    C13 H17 N2 O14 P3 3DLigand Explorer Link out to Ligand Expos:N5P Link out to SuperLigands:N5P Link out to SuperHapten:N5P
     
     
  •  
    Move Section Derived Data Hide
     
     
 
< Biological Assembly 1    Help >
Help | Display Options
Biological assembly 1 assigned by authors
 
 
 
  •  
    Move Section Deposition Summary Hide
    Authors:   Zahn, K.E.,   Belrhali, H.,   Wallace, S.S.,   Doublie, S.

    Deposition:   2007-02-22
    Release:   2007-10-23
    Last Modified (REVDAT):   2009-02-24
     
     
  •  
    Move Section Experimental Details Hide
    Method:   X-RAY DIFFRACTION
    Experimental Data:   Download Structure Factors  [ EDS External Link to EDS ]
    View a histogram of Resolution Resolution[Å]: 2.86
    R-Value: 0.234 (obs.)
    R-Free: 0.294
    Space Group: P 21
    Unit Cell:
      Length [Å] Angles [°]
    a = 132.59 α = 90.00 
    b = 123.48 β = 96.22 
    c = 164.33 γ = 90.00