2L2K

Solution NMR structure of the R/G STEM LOOP RNA-ADAR2 DSRBM2 Complex


Experimental Data Snapshot

  • Method: SOLUTION NMR
  • Conformers Calculated: 200 
  • Conformers Submitted: 20 
  • Selection Criteria: structures with the lowest energy 

wwPDB Validation   3D Report Full Report


This is version 1.1 of the entry. See complete history


Literature

The Solution Structure of the ADAR2 dsRBM-RNA Complex Reveals a Sequence-Specific Readout of the Minor Groove.

Stefl, R.Oberstrass, F.C.Hood, J.L.Jourdan, M.Zimmermann, M.Skrisovska, L.Maris, C.Peng, L.Hofr, C.Emeson, R.B.Allain, F.H.

(2010) Cell 143: 225-237

  • DOI: https://doi.org/10.1016/j.cell.2010.09.026
  • Primary Citation of Related Structures:  
    2L2J, 2L2K, 2L3C, 2L3J

  • PubMed Abstract: 

    Sequence-dependent recognition of dsDNA-binding proteins is well understood, yet sequence-specific recognition of dsRNA by proteins remains largely unknown, despite their importance in RNA maturation pathways. Adenosine deaminases that act on RNA (ADARs) recode genomic information by the site-selective deamination of adenosine. Here, we report the solution structure of the ADAR2 double-stranded RNA-binding motifs (dsRBMs) bound to a stem-loop pre-mRNA encoding the R/G editing site of GluR-2. The structure provides a molecular basis for how dsRBMs recognize the shape, and also more surprisingly, the sequence of the dsRNA. The unexpected direct readout of the RNA primary sequence by dsRBMs is achieved via the minor groove of the dsRNA and this recognition is critical for both editing and binding affinity at the R/G site of GluR-2. More generally, our findings suggest a solution to the sequence-specific paradox faced by many dsRBM-containing proteins that are involved in post-transcriptional regulation of gene expression.


  • Organizational Affiliation

    Institute of Molecular Biology and Biophysics, ETH Zurich, CH-8093 Zürich, Switzerland.


Macromolecules

Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
Adenosine deaminaseA [auth B]71Mus musculusMutation(s): 0 
Gene Names: ADAR2Adarb1mCG_3378RED1
EC: 3.5
UniProt & NIH Common Fund Data Resources
Find proteins for Q91ZS8 (Mus musculus)
Explore Q91ZS8 
Go to UniProtKB:  Q91ZS8
IMPC:  MGI:891999
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupQ91ZS8
Sequence Annotations
Expand
  • Reference Sequence
Find similar nucleic acids by:  (by identity cutoff)  |  3D Structure
Entity ID: 2
MoleculeChains LengthOrganismImage
RNA (42-MER)B [auth A]42Homo sapiens
Sequence Annotations
Expand
  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: SOLUTION NMR
  • Conformers Calculated: 200 
  • Conformers Submitted: 20 
  • Selection Criteria: structures with the lowest energy 

Structure Validation

View Full Validation Report



Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 2010-10-27
    Type: Initial release
  • Version 1.1: 2011-07-13
    Changes: Version format compliance