CRYSTAL STRUCTURE OF THE UBIQUITIN-SPECIFIC PROTEASE ENCODED BY MURINE CYTOMEGALOVIRUS TEGUMENT PROTEIN M48 IN COMPLEX WITH A UBQUITIN-BASED SUICIDE SUBSTRATE     DOI:10.2210/pdb2j7q/pdb

2J7Q Display Files close FASTA Sequence PDB File PDB File (Header) mmCIF File mmCIF File (Header) PDBML/XML File PDBML/XML File (Header) Download Files close FASTA Sequence PDB File (Text) PDB File (gz) mmCIF File mmCIF File (gz) PDBML/XML File PDBML/XML File (gz) Structure Factor (Text) Structure Factor (gz) Biological Assembly 1 (gz) (A+S) Biological Assembly 2 (gz) (A+S) Print this Page Share this Page close Email Delicious Stumble Google More... Link this Page

Primary Citation       Molecular Description Hide Classification: Hydrolase Structure Weight: 68997.25   Molecule: MCMV TEGUMENT PROTEIN M48 ENCODED UBIQUITIN-SPECIFIC PROTEASE, M48USP Polymer: 1 Type: polypeptide(L) Length: 232 Chains: A Other Details: DEUBIQUITINATING MODULE OF MURINE CYTOMEGALOVIRUS TEGUMENT PROTEIN M48. ACTIVE SITE CYSTEINE 23 IS COVALENTLY LINKED TO THE FORMER VINYLMETHYLESTER MOIETY OF THE SUICIDE SUBSTATE UBVME Molecule: UBIQUITIN Polymer: 2 Type: polypeptide(L) Length: 75 Chains: B, D Fragment: UBIQUITIN FUSED TO VINYLMETHYLESTER, UBVME, RESIDUES 1-75 Other Details: THE C-TERMINAL GLY 76 IS REPLACED BY THE VINYLMETHYLESTER MOIETY Molecule: MCMV TEGUMENT PROTEIN M48 ENCODED UBIQUITIN-SPECIFIC PROTEASE, M48USP Polymer: 3 Type: polypeptide(L) Length: 232 Chains: C Other Details: DEUBIQUITINATING MODULE OF MURINE CYTOMEGALOVIRUS TEGUMENT PROTEIN M48. ACTIVE SITE CYSTEINE 23 IS COVALENTLY LINKED TO THE FORMER VINYLMETHYLESTER MOIETY OF THE SUICIDE SUBSTATE UBVME       Source Hide Polymer: 1 Scientific Name: Murine cytomegalovirus Expression System: Escherichia coli   Polymer: 2 Scientific Name: Homo sapiens Common Name: Human Expression System: Escherichia coli   Polymer: 3 Scientific Name: Murine cytomegalovirus Expression System: Escherichia coli         Related PDB Entries Hide Id Details 1C3T  ROTAMER STRAIN AS A DETERMINANT OF PROTEIN STRUCTURALSPECIFICITY  1D3Z  UBIQUITIN NMR STRUCTURE  1F9J  STRUCTURE OF A NEW CRYSTAL FORM OF TETRAUBIQUITIN  1FXT  STRUCTURE OF A CONJUGATING ENZYME-UBIQUITIN THIOLESTERCOMPLEX  1G6J  STRUCTURE OF RECOMBINANT HUMAN UBIQUITIN IN AOT REVERSEMICELLES  1GJZ  SOLUTION STRUCTURE OF A DIMERIC N-TERMINAL FRAGMENT OF HUMAN UBIQUITIN  1NBF  CRYSTAL STRUCTURE OF A UBP-FAMILY DEUBIQUITINATING ENZYMEIN ISOLATION AND IN COMPLEX WITH UBIQUITIN ALDEHYDE  1OGW  SYNTHETIC UBIQUITIN WITH FLUORO-LEU AT 50 AND 67  1Q5W  UBIQUITIN RECOGNITION BY NPL4 ZINC-FINGERS  1S1Q  TSG101(UEV) DOMAIN IN COMPLEX WITH UBIQUITIN  1SIF  CRYSTAL STRUCTURE OF A MULTIPLE HYDROPHOBIC CORE MUTANT OFUBIQUITIN  1TBE  TETRAUBIQUITIN  1UBI  UBIQUITIN  1UBQ  UBIQUITIN  1XD3  CRYSTAL STRUCTURE OF UCHL3-UBVME COMPLEX  1XQQ  SIMULTANEOUS DETERMINATION OF PROTEIN STRUCTURE AND DYNAMICS  1YX5  SOLUTION STRUCTURE OF S5A UIM-1/UBIQUITIN COMPLEX  1YX6  SOLUTION STRUCTURE OF S5A UIM-2/UBIQUITIN COMPLEX  1ZGU  SOLUTION STRUCTURE OF THE HUMAN MMS2- UBIQUITIN COMPLEX  2AYO  STRUCTURE OF USP14 BOUND TO UBQUITIN ALDEHYDE  2BGF  NMR STRUCTURE OF LYS48-LINKED DI-UBIQUITIN USING CHEMICAL SHIFT PERTURBATION DATA TOGETHER WITH RDCS AND 15N-RELAXATION DATA  2FCM  X-RAY CRYSTAL STRUCTURE OF A CHEMICALLY SYNTHESIZED [D-GLN35]UBIQUITIN WITH A CUBIC SPACE GROUP  2FCN  X-RAY CRYSTAL STRUCTURE OF A CHEMICALLY SYNTHESIZED [D-VAL35]UBIQUITIN WITH A CUBIC SPACE GROUP  2FCQ  X-RAY CRYSTAL STRUCTURE OF A CHEMICALLY SYNTHESIZEDUBIQUITIN WITH A CUBIC SPACE GROUP  2FCS  X-RAY CRYSTAL STRUCTURE OF A CHEMICALLY SYNTHESIZED [L-GLN35]UBIQUITIN WITH A CUBIC SPACE GROUP  2FUH  SOLUTION STRUCTURE OF THE UBCH5C/UB NON- COVALENT COMPLEX  2G45  CO-CRYSTAL STRUCTURE OF ZNF UBP DOMAIN FROM THEDEUBIQUITINATING ENZYME ISOPEPTIDASE T (ISOT) IN COMPLEXWITH UBIQUITIN  2GBK  CRYSTAL STRUCTURE OF THE 9-10 MOAD INSERTION MUTANT OFUBIQUITIN  2GBM  CRYSTAL STRUCTURE OF THE 35-36 8 GLYCINE INSERTION MUTANTOF UBIQUITIN  2GBN  CRYSTAL STRUCTURE OF THE 35-36 8 GLYCINE INSERTION MUTANTOF UBIQUITIN        Ligand Chemical Component Hide Identifier Name Formula Interaction View Links GOL     GLYCEROL C3 H8 O3 Ligand Explorer GVE     METHYL 4-AMINOBUTANOATE C5 H11 N O2 Ligand Explorer MG     MAGNESIUM ION Mg Ligand Explorer PG4     TETRAETHYLENE GLYCOL C8 H18 O5 Ligand Explorer       Modified Residues Hide Identifier Name Formula Interaction View Links MSE SELENOMETHIONINE (parent: MET)(type: lPeptideLinking) C5 H11 N O2 Se Ligand Explorer       Derived Data Hide SCOP Classification v1.75 - (4 Domains) CATH Classification v3.2.0 - (2 Domains) PFAM Classification - (2 Domains) GO Terms - (21 Terms)     Reset Layout

Biological Assembly 1     More Images... View in Jmol Other Viewers close KiNG WebMol QuickPDB FirstGlance Kiosk SimpleViewer Protein Workshop Ligand Explorer Biological assembly 1 assigned by authors and generated by PQS (software) Biological assembly 2 assigned by authors and generated by PQS (software)         Deposition Summary Hide Authors:   Schlieker, C.,   Weihofen, W.A.,   Frijns, E.,   Kattenhorn, L.M.,   Gaudet, R.,   Ploegh, H.L. Deposition:   2006-10-16 Release:   2007-03-20 Last Modified (REVDAT):   2009-02-24       Experimental Details Hide Method:   X-RAY DIFFRACTION Experimental Data:    [ EDS ] Resolution[Å]: 1.80 R-Value: 0.157 (obs.) R-Free: 0.214 Space Group: P 1 Unit Cell:   Length [Å] Angles [°] a = 40.91 α = 73.37  b = 57.30 β = 85.37  c = 67.28 γ = 88.54        Structure Links Hide Molecule of the Month Features: Ubiquitin