1ZIH

GCAA RNA TETRALOOP, NMR, 10 STRUCTURES


Experimental Data Snapshot

  • Method: SOLUTION NMR
  • Conformers Submitted: 10 

wwPDB Validation   3D Report Full Report


This is version 1.3 of the entry. See complete history


Literature

A network of heterogeneous hydrogen bonds in GNRA tetraloops.

Jucker, F.M.Heus, H.A.Yip, P.F.Moors, E.H.Pardi, A.

(1996) J Mol Biol 264: 968-980

  • DOI: https://doi.org/10.1006/jmbi.1996.0690
  • Primary Citation of Related Structures:  
    1ZIF, 1ZIG, 1ZIH

  • PubMed Abstract: 

    RNA hairpin loops containing a GNRA consensus sequence are the most frequently occurring hairpins in a variety of prokaryotic and eukaryotic RNAs. These tetraloops play important functional roles in RNA folding, in RNA-RNA tertiary interactions and as protein binding sites. Homo and heteronuclear NMR spectroscopy have been used to determine the structures of the most abundant members of the GNRA tetraloop family: the GAGA, GCAA and GAAA loops closed by a C-G base pair. Analysis of the structures of these three hairpin loops reveals a network of heterogeneous hydrogen bonds. The loops contain a G-A base pair, a G base-phosphate hydrogen bond and several 2' OH-base hydrogen bonds. These intramolecular interactions and the extensive base stacking in the loop help explain the high thermodynamic stability and give insight into the diverse biological roles of the GNRA RNA hairpins.


  • Organizational Affiliation

    Department of Chemistry and Biochemistry, University of Colorado at Boulder, 80309-0215, USA.


Macromolecules

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Entity ID: 1
MoleculeChains LengthOrganismImage
RNA (5'-R(*GP*GP*GP*CP*GP*CP*AP*AP*GP*CP*CP*U)-3')12N/A
Sequence Annotations
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: SOLUTION NMR
  • Conformers Submitted: 10 

Structure Validation

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Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 1997-03-12
    Type: Initial release
  • Version 1.1: 2008-03-24
    Changes: Version format compliance
  • Version 1.2: 2011-07-13
    Changes: Version format compliance
  • Version 1.3: 2022-03-02
    Changes: Database references, Derived calculations, Other